ab72957, at 10µg/ml, staining QARS in HeLa cells by immunofluorescence.
Immunoprecipitation - QARS antibody (ab72957)Image courtesy of an anonymous Abreview.
Cells were lysed hypotonically in 10 mM HEPES pH 7.6, 10 mM KOAc, 1.5 mM Mg(OAc)2, 0.5 mM DTT followed by douncing, followed by salt adjustment to 130 mM KOAc and addition of 1% NP40 for immunoprecipitation. Lane 1: inputLane 2: supernatant, control antibody Lane 3: supernatant, ab72957 at a 1/50 dilution.Lane 4: beads. control antibody Lane 5: beads, ab72957 at a 1/50 dilution. Whole cell lysate prepared from HeLa cells was loaded at 25000000 cells. The immunoprecipitation step was performed using Protein G. For WB ab72957 used at a 1/1000 dilution.