製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to QARS
  • アプリケーション
    適用あり: WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human QARS protein (NP_005042.1, 1 a.a. ~ 775 a.a).

  • ポジティブ・コントロール
    • Human liver tissue lysate. QARS transfected 293T cell lysate. HeLa cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 88 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.

ターゲット情報

  • 配列類似性
    Belongs to the class-I aminoacyl-tRNA synthetase family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GlnRS antibody
    • Glutamine tRNA ligase antibody
    • Glutamine tRNA synthetase antibody
    • Glutamine--tRNA ligase antibody
    • Glutaminyl tRNA ligase antibody
    • Glutaminyl tRNA synthetase antibody
    • Glutaminyl-tRNA synthetase antibody
    • PRO2195 antibody
    • QARS antibody
    • SYQ_HUMAN antibody
    see all

Anti-QARS antibody 画像

  • Anti-QARS antibody (ab72957) at 1 µg/ml + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • All lanes : Anti-QARS antibody (ab72957) at 1 µg/ml

    Lane 1 : QARS transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • ab72957, at 10µg/ml, staining QARS in HeLa cells by immunofluorescence.
  • Cells were lysed hypotonically in 10 mM HEPES pH 7.6, 10 mM KOAc, 1.5 mM Mg(OAc)2, 0.5 mM DTT followed by douncing, followed by salt adjustment to 130 mM KOAc and addition of 1% NP40 for immunoprecipitation.
    Lane 1: input
    Lane 2: supernatant, control antibody
    Lane 3: supernatant, ab72957 at a 1/50 dilution.
    Lane 4: beads. control antibody
    Lane 5: beads, ab72957 at a 1/50 dilution.

    Whole cell lysate prepared from HeLa cells was loaded at 25000000 cells. The immunoprecipitation step was performed using Protein G. For WB ab72957 used at a 1/1000 dilution.

    See Abreview

Anti-QARS antibody (ab72957) 使用論文

ab72957 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
2.5e+007 cells
Specification
HeLa
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

投稿 Jul 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell)
Loading amount
10 µg
Specification
HeLa cell
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (RAJI B-Cells)
Loading amount
20 µg
Specification
RAJI B-Cells
Gel Running Conditions
Reduced Denaturing (12% Tris-HEPES-SDS)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録