Anti-Pyruvate Kinase 抗体 (HRP) - Azide free (ab34554)

製品の概要

  • 製品名Anti-Pyruvate Kinase antibody (HRP) - Azide free
    Pyruvate Kinase 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Pyruvate Kinase - Azide free (HRP)
  • 標識HRP
  • アプリケーション適用あり: WB, ELISA, Electron Microscopy, Dot blotmore details
  • 種交差性
    交差種: Rabbit
  • 免疫原

    Full length native protein (purified): Pyruvate Kinase from Rabbit muscle

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab34554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
Electron Microscopy
Dot blot
  • 追加情報Dot: Use at an assay dependent dilution.
    Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/15000 - 1/60000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 58 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Pyruvate kinase is also known as ATP:pyruvate phosphotransferase. There are 4 isoforms of piruvate kinase in mammals: L, R, M1 and M2. L type is the major isoform in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells. Defects in PKLR (pyruvate kinase L and R) are the cause of pyruvate kinase hyperactivity; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP. Also defects in PKLR are a cause of chronic nonspherocytic hemolytic anemia (CNSHA); also called hereditary nonspherocytic hemolytic anemia.
    • 細胞内局在Cytoplasmic
    • 参照データベース
    • 別名
      • Pyruvate kinase 3 antibody
      • CTHBP antibody
      • Cytosolic thyroid hormone-binding protein antibody
      • epididymis secretory protein Li 30 antibody
      • HEL-S-30 antibody
      • OIP3 antibody
      • OPA interacting protein 3 antibody
      • p58 antibody
      • PK, muscle type antibody
      • PK1 antibody
      • PK2 antibody
      • PKL antibody
      • PKLR antibody
      • PKM 2 antibody
      • PKM antibody
      • PKM2 antibody
      • PYKM antibody
      • Pyruvate kinase 1 antibody
      • Pyruvate kinase 2/3 antibody
      • Pyruvate kinase isozyme R/L antibody
      • pyruvate kinase isozymes L/R antibody
      • Pyruvate kinase isozymes M1/M2 antibody
      • pyruvate kinase isozymes R/L antibody
      • Pyruvate kinase liver and blood cell antibody
      • Pyruvate kinase liver and RBC antibody
      • Pyruvate kinase muscle antibody
      • Pyruvate kinase muscle isozyme antibody
      • pyruvate kinase PKM antibody
      • pyruvate kinase type L antibody
      • Pyruvate kinase, liver and red blood cell antibody
      • Pyruvate kinase, liver type antibody
      • Pyruvate kinase, muscle, 2 antibody
      • Pyruvate kinase, red cell type antibody
      • R type/L type pyruvate kinase antibody
      • Red cell/liver pyruvate kinase antibody
      • RPK antibody
      • TCB antibody
      • THBP1 antibody
      • Thyroid hormone binding protein cytosolic antibody
      • thyroid hormone-binding protein 1 antibody
      • tumor M2-PK antibody
      see all

    Anti-Pyruvate Kinase antibody (HRP) - Azide free (ab34554) 使用論文

    ab34554 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 100 cells
    Gel Running Conditions Reduced Denaturing (8%)
    Sample Mouse Cell lysate - whole cell (Bone marrow-derived macrophages)
    Specification Bone marrow-derived macrophages
    Treatment poly I:C 1 ug/ml for the indicated times (0, 6, 24 and 48 hours)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 04 2015

    Thank you for contacting us.

    Please see below for the anwers to your questions:

    ab34554: This antibody has only been tested with rabbit samples so far. We do not know how well it might work with human samples, but the homology is ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"