Anti-Pyruvate dehydrogenase E2/E3bp 抗体 [13G2AE2BH5] (ab110333)

製品の概要

  • 製品名
    Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5]
  • 製品の詳細
    Mouse monoclonal [13G2AE2BH5] to Pyruvate dehydrogenase E2/E3bp
  • アプリケーション
    適用あり: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Porcine Pyruvate dehydrogenase E2/E3bp.

  • ポジティブ・コントロール
    • Isolated mitochondria from Human, Bovine, Rat and Mouse heart and HepG2 lysate; cultured, normal Human embryonic lung fibroblasts (strain MRC5); Human cerebellum tissue; HL60 cells.
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 , 69 kDa.
ICC/IF Use a concentration of 1 µg/ml. (heat-induced antigen-retrieval improves signal)
Flow Cyt Use a concentration of 1 mg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • 関連疾患
    Note=Primary biliary cirrhosis is a chronic, progressive cholestatic liver disease characterized by the presence of antimitochondrial autoantibodies in patients' serum. It manifests with inflammatory obliteration of intra-hepatic bile duct, leading to liver cell damage and cirrhosis. Patients with primary biliary cirrhosis show autoantibodies against the E2 component of pyruvate dehydrogenase complex.
    Defects in DLAT are the cause of pyruvate dehydrogenase E2 deficiency (PDHE2 deficiency) [MIM:245348]; also known as lactic acidemia due to defect of E2 lipoyl transacetylase of the pyruvate dehydrogenase complex. Pyruvate dehydrogenase (PDH) deficiency is a major cause of primary lactic acidosis and neurological dysfunction in infancy and early childhood. In this form of PDH deficiency episodic dystonia is the major neurological manifestation, with other more common features of pyruvate dehydrogenase deficiency, such as hypotonia and ataxia, being less prominent.
  • 配列類似性
    Belongs to the 2-oxoacid dehydrogenase family.
    Contains 2 lipoyl-binding domains.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody
    • AI481367 antibody
    • BOS_15094 antibody
    • Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody
    • Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex antibody
    • DLAT antibody
    • DLDBP antibody
    • DLTA antibody
    • E3BP antibody
    • M2 antigen complex 70 kDa subunit antibody
    • MGC143185 antibody
    • mitochondrial antibody
    • ODP2_HUMAN antibody
    • OPDX antibody
    • PBC antibody
    • PDC-E2 antibody
    • PDCE2 antibody
    • PDX1 antibody
    • proX antibody
    • Pyruvate dehydrogenase complex component E2 antibody
    • Pyruvate dehydrogenase complex, component X antibody
    • RP23-203N8.2 antibody
    see all

画像

  • ab110333 staining Pyruvate dehydrogenase E2/E3bp in Human HUVEC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/1000 in PBS) for 1 hour. A CF568-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : HepG2 cell lysate at 20 µg


    Predicted band size : 54 , 69 kDa
  • Immunocytochemistry analysis using ab110333 at 1µg/ml staining Pyruvate dehydrogenase E2/E3bp in cultured, normal Human embryonic lung fibroblasts and an AlexaFluor® 488 goat anti-mouse IgG2a secondary antibody (2 ug/ml).
  • Immunohistological analysis using ab110333 at 1/1000 dilution staining Pyruvate dehydrogenase E2/E3bp in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
    Note: Immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
  • Flow cytometric analysis using ab110333 at 1µg/ml staining Pyruvate dehydrogenase E2/E3b in HL60 cells (blue). Isotype control antibody (red).

参考文献

This product has been referenced in:
  • Yamada T  et al. Dynamin-Related Protein 1 Deficiency Leads to Receptor-Interacting Protein Kinase 3-Mediated Necroptotic Neurodegeneration. Am J Pathol 186:2798-2802 (2016). Read more (PubMed: 27640145) »
  • Koukourakis MI  et al. Normal tissue radioprotection by amifostine via Warburg-type effects. Sci Rep 6:30986 (2016). WB . Read more (PubMed: 27507219) »

See all 14 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (H9C2)
Permeabilization
Yes - 0.1% Triton in PBS for 10 min
Specification
H9C2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 17 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing (10% acrylamide gel)
Loading amount
20 µg
Specification
prostate cancer cell line
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Stamatia Pouliliou

Verified customer

投稿 Jun 26 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (HuVEC)
Specification
HuVEC
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

投稿 Nov 18 2013

Thank you for your reply.

The kit ab109902 measures the activity of the whole PDH complex by assaying generated NADH. This requires all three activities (pyruvate dehydrogenase –E1, dihydrolipoyl acyltransferase-E2 and dihydrolipoyl deh...

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Thank you for contacting us.

I have had the opportunity to speak with the lab regarding this on your behalf. They recommend using ab110333 (MSP06) with rat samples. The antibody targets on E2/E3 binding protein but pulls down the whole PDH c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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