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Synthetic peptide: LLQLLGSPGR RYYSLPPHQK VPLPSLSPTM QAGTIARWEK KEGDKINEGD, corresponding to a region within N terminal amino acids 74-123 of Human Pyruvate Dehydrogenase E2
Our Abpromise guarantee covers the use of ab66511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|IHC-FoFr||Use at an assay dependent concentration.|
Immunochemistry/Immunofluorescence analysis of Hek293 cell labelling Pyruvate Dehydrogenase E2 with ab66511 at 1/100 dilution. An anti-rabbit Alexa Fluor 488 was used as the secondary antibody at 1/400 dilution. Mitotraker-Red was used to stain mitochondria and DAPI was used to stain nuclei.
This image is courtesy of an anonymous AbreviewBlocking Step: 5% Milk for 1 hour at 25°C.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Pyruvate Dehydrogenase E2 with ab66511 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.