Anti-Pyruvate Dehydrogenase E1-alpha subunit 抗体 (ab67592)

製品の概要

  • 製品名
    Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody
    Pyruvate Dehydrogenase E1-alpha subunit 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Pyruvate Dehydrogenase E1-alpha subunit
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length PDHA1 protein (Human)

  • ポジティブ・コントロール
    • Human liver tissue lysate, PDHA1 transfected lysate and Human stomach tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P Use a concentration of 1.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • 組織特異性
    Ubiquitous.
  • 関連疾患
    Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
    Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ODPA_HUMAN antibody
    • PDH antibody
    • PDHA antibody
    • PDHA1 antibody
    • PDHCE1A antibody
    • PDHE1 A type I antibody
    • PDHE1-A type I antibody
    • PHE1A antibody
    • Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody
    • Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody
    • Pyruvate Dehydrogenase E1 alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody
    see all

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody 画像

  • Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution + Human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution

    Lane 1 : Pyruvate Dehydrogenase E1-alpha subunit transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of formalin fixed and paraffin embedded human stomach tissue labelled with ab67592 at 1.5µg/ml.
  • ICC/IF image of ab67592 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67592, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) 使用論文

This product has been referenced in:

See all 5 Publications for this product

Product Wall

Thank you for confirming those details.

I can suggest the goat anti-mouse antibodyhttp://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98808.htmlor the rabbit anti-mouse antibodyhttp://www.abcam.com/Rabb...

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Thank you for contacting us and your interest in our products.

I am sorry but I have not completely understood the question. If you are asking if ab99823 can be used as a secondary antibody to ab67592 and ab52587 then the answer would be no. ...

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Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Alfredo Castello

Verified customer

投稿 Aug 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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