Anti-Pyruvate Dehydrogenase E1-alpha subunit 抗体 (ab110474)

製品の概要

  • 製品名Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody
    Pyruvate Dehydrogenase E1-alpha subunit 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Pyruvate Dehydrogenase E1-alpha subunit
  • 特異性ab110474 detects endogenous levels of total Pyruvate Dehydrogenase E1-alpha subunit protein.
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal region of Human Pyruvate Dehydrogenase E1-alpha subunit (UniProt ID: P08559).

  • ポジティブ・コントロール
    • WB: Extracts from A549, HepG2 and Jurkat cells IHC-P: Human breast carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval is heat-mediated, citrate buffer pH 6.0. A pressure cooker was used for about 5 minutes.

ターゲット情報

  • 機能The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • 組織特異性Ubiquitous.
  • 関連疾患Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
    Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ODPA_HUMAN antibody
    • PDH antibody
    • PDHA antibody
    • PDHA1 antibody
    • PDHCE1A antibody
    • PDHE1 A type I antibody
    • PDHE1-A type I antibody
    • PHE1A antibody
    • Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody
    • Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody
    • Pyruvate Dehydrogenase E1 alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody
    see all

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody 画像

  • All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab110474) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from A549 cells
    Lane 4 : Extracts from Jurkat cells, treated with the synthesized peptide

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 43 kDa
  • ab110474, at a 1/50 dilution, staining Pyruvate Dehydrogenase E1-alpha subunit in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the synthesized peptide.

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab110474) 使用論文

ab110474 has not yet been referenced specifically in any publications.

Product Wall

Yes, ab110474 was run under reduced, denatured conditions.

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Mouse Tissue sections (mouse brain tissue sections)
Specification mouse brain tissue sections
Permeabilization Yes - 0.3% of triton-X 100
Fixative Paraformaldehyde
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Qian Ma

Verified customer

投稿 Jan 03 2014

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