Anti-Pyruvate Dehydrogenase E1-alpha subunit 抗体 [9H9AF5] (ab110330)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF Use a concentration of 5 µg/ml. (heat-induced antigen-retrieval improves signal)
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

In-Cell ELISA Use a concentration of 8 µg/ml.

ターゲット情報

  • 機能The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • 組織特異性Ubiquitous.
  • 関連疾患Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
    Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ODPA_HUMAN antibody
    • PDH antibody
    • PDHA antibody
    • PDHA1 antibody
    • PDHCE1A antibody
    • PDHE1 A type I antibody
    • PDHE1-A type I antibody
    • PHE1A antibody
    • Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody
    • Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody
    • Pyruvate Dehydrogenase E1 alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody
    see all

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [9H9AF5] 画像

  • Immunocytochemistry analysis using ab110330 at 5µg/ml staining Pyruvate Dehydrogenase E1-alpha subunit in cultured, normal Human embryonic lung fibroblasts (strain MRC5) followed by AlexaFluor® 488 goat anti-mouse IgG1 secondary antibody (2 µg/ml).
  • Immunocytochemical analysis of SH-SY5Y human neuroblastoma labeling Pyruvate dehydrogenase E1-alpha with ab110330 at 1/500 dilution.

    See Abreview

  • Flow cytometric analysis using ab110330 at 1µg/ml staining Pyruvate Dehydrogenase E1-alpha subunit in HL60 cells (blue). Isotype control antibody (red).
  • All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [9H9AF5] (ab110330) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 10 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 4 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : hepG2 cell lysate at 20 µg


    Predicted band size : 43 kDa

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [9H9AF5] (ab110330) 使用論文

This product has been referenced in:
  • Zhang Y  et al. Induction of Posttranslational Modifications of Mitochondrial Proteins by ATP Contributes to Negative Regulation of Mitochondrial Function. PLoS One 11:e0150454 (2016). Mouse . Read more (PubMed: 26930489) »
  • Vavrova E  et al. Muscle expression of a malonyl-CoA-insensitive carnitine palmitoyltransferase-1 protects mice against high-fat/high-sucrose diet-induced insulin resistance. Am J Physiol Endocrinol Metab 311:E649-60 (2016). Read more (PubMed: 27507552) »

See all 20 Publications for this product

Product Wall

Thank you for your enquiry,

I am sorry to confirm that this kit is no longer available, and apologise for the inconvenience.

I can confirm that we do sell the separate antibody components which you may like to consider:

Read More

Application Western blot
Sample Sheep Tissue lysate - whole (liver)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Jennifer Bruce

Verified customer

投稿 Jun 12 2015

Application Western blot
Sample Human Cell lysate - whole cell (Ovarian cancer cell lines)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 10 µg
Specification Ovarian cancer cell lines
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 May 25 2015

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Mouse Tissue lysate - whole (heart)
Specification heart
Total protein in input 1000 µg
Username

Abcam user community

Verified customer

投稿 Feb 23 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Human Cell (SH-SY5Y human neuroblastoma)
Specification SH-SY5Y human neuroblastoma
Permeabilization Yes - 0.2% NP-40, 3min
Fixative Formaldehyde
Username

Dr. George Allen

Verified customer

投稿 Dec 16 2013

Thank you for contacting us.

Please find the WB protocol used to validate our MitoSciences range of antibodies attached, as well as the pdf datasheets for those products. Keep in mind, we regularly update our online datasheets to reflect the ...

Read More

Thank you for your enquiry. It has taken some time to confirm all the details for you, and I appreciate your patience.

I am sorry to confirm that MS602 has been discontinued and we do not have anymore in stock.

Although that produ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"