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Pyruvate Assay Kit (Colorimetric/Fluorometric) (ab65342) provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc. In the assay, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (?= 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate concentration can be accurately measured. The kit detects 1-200 µM pyruvate.
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Pyruvate is a central molecule in metabolism through which sugars enter the citric acid cycle. Pyruvate can be converted to carbohydrates during gluconeogenesis or to fatty acids via acetyl CoA. High levels of pyruvate are associated with liver disease and genetic disorders. Pyruvate has also been used to stimulate metabolism leading to loss of body weight.
|Pyruvate Assay Buffer||WM||1 x 25ml|
|Pyruvate Enzyme Mix (Lyophilized)||Green||1 vial|
|Pyruvate Probe (in DMSO)||Red||1 x 200µl|
|Pyruvate Standard (100 nmol/µl)||Yellow||1 x 100µl|
Our Abpromise guarantee covers the use of ab65342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Pyruvate measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
Pyruvate measured colourimetrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
Pyruvate measured fluorometrically in biological fluids showing quantity (nmol) per mln of tested cells
Pyruvate measured fluorometrically in biological fluids showing quantity (nmol) per mL of tested sample
Pyruvate measured colourimetrically in biological fluids showing quantity (nmol) per mL of tested sample
Pyruvate colorimetric standard curve.
Pyruvate fluorometric standard curve.