製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PYROXD1
  • アプリケーション
    適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: QLATHFPSEDILLVTASPVIKAVTNFKQISKILEEFDVEEQSSTMLGKRF PNIKVIESGVKQLK SEEHCIVTEDGNQHVYKKLCLCAGAKPKLICEGN, corresponding to amino acids 28-125 of Human PYROXD1.

  • ポジティブ・コントロール
    • Human colon tissue; RT-4 cell lysate; Human liver lysate
  • 特記事項


    ab122458 is mono-specific.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    ab122458 is mono-specific.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 配列類似性
    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. PYROXD1 subfamily.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp762G094 antibody
    • FLJ22028 antibody
    • OTTHUMP00000240138 antibody
    • OTTHUMP00000240140 antibody
    • PYRD1_HUMAN antibody
    • Pyridine nucleotide disulphide oxidoreductase domain 1 antibody
    • Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1 antibody
    • PYROXD 1 antibody
    • PYROXD1 antibody
    see all

画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122458 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-PYROXD1 antibody (ab122458) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate
  • ab122458, at 1/50 dilution, staining PYROXD1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

参考文献

This product has been referenced in:
  • O'Grady GL  et al. Variants in the Oxidoreductase PYROXD1 Cause Early-Onset Myopathy with Internalized Nuclei and Myofibrillar Disorganization. Am J Hum Genet 99:1086-1105 (2016). Read more (PubMed: 27745833) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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