製品の概要

  • 製品名Anti-PYK2 (phospho Y402) antibody
    PYK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PYK2 (phospho Y402)
  • アプリケーション適用あり: ICC/IF, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosine 402. The sequence is conserved in human and rat.

  • ポジティブ・コントロール
    • Chick embryo fibroblast (CEF) cells expressing human PYK 2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
IHC-Fr
  • 追加情報IHC-Fr: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 116 kDa. Can be blocked with PYK2 peptide - phospho Y402 (phospho and non-phospho pair) or CEF whole cell lysate, positive / negative for PYK 2 (pair).
    ICC/IF: Use at an assay dependent dilution from (PubMed:17325207).
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
    • 組織特異性Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
    • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
    • 細胞内局在Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CADTK antibody
      • CAK-beta antibody
      • CAKB antibody
      • CAKbeta antibody
      • Calcium regulated non receptor proline rich tyrosine kinase antibody
      • Calcium-dependent tyrosine kinase antibody
      • Cell adhesion kinase beta antibody
      • E430023O05Rik antibody
      • EC 2.7.10.2 antibody
      • FADK 2 antibody
      • FADK2 antibody
      • FAK2 antibody
      • FAK2_HUMAN antibody
      • Focal adhesion kinase 2 antibody
      • MGC124628 antibody
      • PKB antibody
      • Proline-rich tyrosine kinase 2 antibody
      • Protein kinase B antibody
      • Protein Tyrosine Kinase 2 Beta antibody
      • Protein-tyrosine kinase 2-beta antibody
      • PTK antibody
      • PTK2B antibody
      • PTK2B protein tyrosine kinase 2 beta antibody
      • PYK2 antibody
      • RAFTK antibody
      • RAFTK2 antibody
      • Related adhesion focal tyrosine kinase antibody
      see all

    Anti-PYK2 (phospho Y402) antibody 画像



    • Predicted band size : 116 kDa


      Peptide Competition: Extracts prepared from CEF cells expressing Pyk2 and plated on fibronectin were resolved by SDS-PAGE on a 10% Tris glycine gel. The proteins were then transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4800 antibody, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to phosphopeptide immunogen (2), a generic phosphotyrosine containing peptide (3), the phosphopeptide corresponding to tyrosine 397 on FAK (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, therefore demonstrating the specificity of ab4800 antibody for this phosphorylated residue.
    • Figure shows Pyk2 staining: Normal mouse mammary (NmuMG) cells were treated with TGFbeta and then grown to confluence, fixed and permeabilized. ab4800 was identified using a selective PSSA followed by incubation with a Cy3-conjugated anti-rabbit secondary antibody (red). F actin was visualized with Oregon Green-conjugated phalloiden (green) and paxillin staining is indicated in blue. The merged image indicates colocalization (purple) of ab4800, F-actin and paxillin in focal adhesion structures.

    Anti-PYK2 (phospho Y402) antibody (ab4800) 使用論文

    This product has been referenced in:
    • Haidari M  et al. Disruption of endothelial adherens junctions by high glucose is mediated by protein kinase C-ß-dependent vascular endothelial cadherin tyrosine phosphorylation. Cardiovasc Diabetol 13:112 (2014). Human . Read more (PubMed: 25027642) »
    • Haidari M  et al. Atorvastatin preserves the integrity of endothelial adherens junctions by inhibiting vascular endothelial cadherin tyrosine phosphorylation. Exp Cell Res 318:1673-84 (2012). WB ; Human . Read more (PubMed: 22659162) »

    See all 5 Publications for this product

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    Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you.

    As we discussed over the phone, I have looked into how the Anti-PYK2 (phospho Y402) antibodies ab4800 and ab131543 have been charac...

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    Thank you for contacting us. I do want to apologize for the tardiness of this response.

    I have been in contact with the originator labs for ab42622 and ab60878 PYK2 proteins, in regards to whether these products are phosphorylated at Y402. U...

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    Thank you for your enquiry. Ab4800 was tested in IHC cryostat sections in the following reference: Melendez J. et al (2002) J. Biol. Chem. 277(47):45203-45210. The authors prepared mouse heart sections fixed in 4% paraformaldehyde/PBS overnight a...

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