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Synthetic peptide within Rat PYK2 aa 950-1050 (C terminal). The exact sequence is proprietary.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab32448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: PYK2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: Hu brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32448 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32448 was shown to specifically react with PYK2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when PYK2 knockout samples were used. Wild-type and PYK2 knockout samples were subjected to SDS-PAGE. Ab32448 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab32448 has not yet been referenced specifically in any publications.