製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PYK2
  • 特異性
    Detects endogenous levels of total PYK2 protein
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human PYK2 around the phosphorylation site of Tyrosine 580 (DYYKA).

  • ポジティブ・コントロール
    • Extracts from K562 cells treated with UV (15 mins); human brain tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 116 kDa.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
  • 組織特異性
    Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
  • 細胞内局在
    Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CADTK antibody
    • CAK-beta antibody
    • CAKB antibody
    • CAKbeta antibody
    • Calcium regulated non receptor proline rich tyrosine kinase antibody
    • Calcium-dependent tyrosine kinase antibody
    • Cell adhesion kinase beta antibody
    • E430023O05Rik antibody
    • EC 2.7.10.2 antibody
    • FADK 2 antibody
    • FADK2 antibody
    • FAK2 antibody
    • FAK2_HUMAN antibody
    • Focal adhesion kinase 2 antibody
    • MGC124628 antibody
    • PKB antibody
    • Proline-rich tyrosine kinase 2 antibody
    • Protein kinase B antibody
    • Protein Tyrosine Kinase 2 Beta antibody
    • Protein-tyrosine kinase 2-beta antibody
    • PTK antibody
    • PTK2B antibody
    • PTK2B protein tyrosine kinase 2 beta antibody
    • PYK2 antibody
    • RAFTK antibody
    • RAFTK2 antibody
    • Related adhesion focal tyrosine kinase antibody
    see all

画像

  • All lanes : Anti-PYK2 antibody (ab55358) at 1/500 dilution

    Lane 1 : extracts from K562 cells treated with UV (15 mins) with no immunizing peptide
    Lane 2 : extracts from K562 cells treated with UV (15 mins) with immunizing peptide


    Predicted band size : 116 kDa
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab55358 at 1/50 dilution. Left: without immunizing peptide; Right: with immunizing peptide

参考文献

This product has been referenced in:
  • Hinterseher I  et al. Immunohistochemical analysis of the natural killer cell cytotoxicity pathway in human abdominal aortic aneurysms. Int J Mol Sci 16:11196-212 (2015). Read more (PubMed: 25993291) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (Sf21 insect cells)
Loading amount
5 µg
Specification
Sf21 insect cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris with MOPS)
Blocking step
LICOR as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
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投稿 Mar 27 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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