製品の概要

  • 製品名Anti-Pygopus 2 antibody [EPR2024(2)]
    Pygopus 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2024(2)] to Pygopus 2
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Pygopus 2.

  • ポジティブ・コントロール
    • MCF7, HeLa, Human ovary cancer, and NCCIT cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    Anti-Pygopus 2 antibody [EPR2024(2)] 画像

    • All lanes : Anti-Pygopus 2 antibody [EPR2024(2)] (ab109001) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human ovary cancer lysate
      Lane 4 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 41 kDa

    Anti-Pygopus 2 antibody [EPR2024(2)] (ab109001) 使用論文

    This product has been referenced in:
    • Li B  et al. Repurposing the FDA-approved pinworm drug pyrvinium as a novel chemotherapeutic agent for intestinal polyposis. PLoS One 9:e101969 (2014). WB ; Human . Read more (PubMed: 25003333) »
    • Liu Y  et al. Abnormal expression of Pygopus 2 correlates with a malignant phenotype in human lung cancer. BMC Cancer 13:346 (2013). WB, IHC . Read more (PubMed: 23865714) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (primary myoblast)
    Specification primary myoblast
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative Paraformaldehyde
    Username

    Fabien LE GRAND

    Verified customer

    投稿 Jun 18 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"