製品の概要

  • 製品名Anti-PYGM antibody
    PYGM 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PYGM
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH from the C terminal region of human PYGM.

  • ポジティブ・コントロール
    • WB: 293 cell lysate. IHC-P: Human skeletal muscle.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

ターゲット情報

  • 機能Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
  • 関連疾患Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) [MIM:232600]; also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.
  • 配列類似性Belongs to the glycogen phosphorylase family.
  • 翻訳後修飾Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Glycogen phosphorylase antibody
    • Glycogen phosphorylase muscle form antibody
    • muscle form antibody
    • Muscpho antibody
    • Myophosphorylase antibody
    • Phosphorylase glycogen muscle (McArdle syndrome glycogen storage disease type V) antibody
    • Pygm antibody
    • PYGM_HUMAN antibody
    see all

Anti-PYGM antibody 画像

  • ab63158, at a 1/10 dilution, staining PYGM in formalin fixed, paraffin embedded human skeletal muscle tissue by Immunohistochemistry. ab63158 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
  • All lanes : Anti-PYGM antibody (ab63158) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysates
    Lane 2 : Lysates of 293 cells transiently transfected with PYGM gene.

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 97 kDa
    Observed band size : 97 kDa

Anti-PYGM antibody (ab63158) 使用論文

This product has been referenced in:
  • Nogales-Gadea G  et al. Expression of Glycogen Phosphorylase Isoforms in Cultured Muscle from Patients with McArdle's Disease Carrying the p.R771PfsX33 PYGM Mutation. PLoS One 5: (2010). WB ; Human . Read more (PubMed: 20957198) »

See 1 Publication for this product

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The homology between mouse and human seems to be very high (above 95%) so cross-reactivity with murine PYGM can be expected even for those two antibodies (ab63158 and ab63159) which hav...

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