製品の概要

  • 製品名Anti-PXK antibody
    PXK 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PXK
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Orangutan
  • 免疫原

    Recombinant fragment: PDSVPVDSFP PAPSMAVVAV LESTLSCEAC KNGMPTISRL LQMPLFSDVL LTTSEKPQFK IPTKLKEALR IAKECIEKRL IEEQKQIHQH RRLTRAQSHH, corresponding to amino acids 351-450 of Human PXK (AAH14479) with a proprietory tag.

  • ポジティブ・コントロール
    • PXK transfected 293T cell lysate. Recombinant tagged human PXK fragment.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 65 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity.
  • 組織特異性Widely expressed in all tissues examined except in heart. Isoform 1 is expressed in high levels in the brain, skeletal muscle, spleen and testis. Isoform 7 expression has yet to be demonstrated.
  • 配列類似性Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
    Contains 1 PX (phox homology) domain.
    Contains 1 WH2 domain.
  • ドメインThe protein kinase domain is predicted to be catalytically inactive.
  • 細胞内局在Cytoplasm. Cell membrane. Also associates with the plasma membrane. Isoform 3 is present throughout the cell.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ20335 antibody
    • K-ATPase antibody
    • Modulator of Na antibody
    • Modulator of Na K ATPase antibody
    • Modulator of Na K ATPase long form antibody
    • MONaKA antibody
    • PX domain containing protein kinase like protein antibody
    • PX domain containing serine/threonine kinase antibody
    • PX domain-containing protein kinase-like protein antibody
    • PX ser/thr kinase v2 antibody
    • PX serine/threonine kinase antibody
    • Pxk antibody
    • PXK_HUMAN antibody
    see all

Anti-PXK antibody 画像

  • Anti-PXK antibody (ab77652) at 1 µg/ml + Recombinant tagged human PXK fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 65 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PXK antibody (ab77652) at 1 µg/ml

    Lane 1 : PXK transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 65 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

Anti-PXK antibody (ab77652) 使用論文

ab77652 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"