製品の概要

  • 製品名
    Anti-PXK antibody
  • 製品の詳細
    Rabbit polyclonal to PXK
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the center-terminal region of human PXK.

  • ポジティブ・コントロール
    • Hela cell line lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Detects bands of approximately 50 and 75 kDa (predicted molecular weight: 44 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity.
    • 組織特異性
      Widely expressed in all tissues examined except in heart. Isoform 1 is expressed in high levels in the brain, skeletal muscle, spleen and testis. Isoform 7 expression has yet to be demonstrated.
    • 配列類似性
      Belongs to the protein kinase superfamily.
      Contains 1 protein kinase domain.
      Contains 1 PX (phox homology) domain.
      Contains 1 WH2 domain.
    • ドメイン
      The protein kinase domain is predicted to be catalytically inactive.
    • 細胞内局在
      Cytoplasm. Cell membrane. Also associates with the plasma membrane. Isoform 3 is present throughout the cell.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ20335 antibody
      • K-ATPase antibody
      • Modulator of Na antibody
      • Modulator of Na K ATPase antibody
      • Modulator of Na K ATPase long form antibody
      • MONaKA antibody
      • PX domain containing protein kinase like protein antibody
      • PX domain containing serine/threonine kinase antibody
      • PX domain-containing protein kinase-like protein antibody
      • PX ser/thr kinase v2 antibody
      • PX serine/threonine kinase antibody
      • Pxk antibody
      • PXK_HUMAN antibody
      see all

    画像

    • Anti-PXK antibody (ab71397) at 1/60 dilution + Hela cell line lysate at 35 µg

      Predicted band size : 44 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    プロトコール

    参考文献

    ab71397 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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