製品の概要

  • 製品名Anti-PVRL1 antibody
  • 製品の詳細
    Rabbit polyclonal to PVRL1
  • アプリケーション適用あり: IHC-FoFr, WB, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human PVRL1.

    (Peptide available as ab73475.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Brain (Mouse) Tissue; Testis (Human) Tissue; HeLa Whole Cell; SK N SH Whole Cell; Hela Whole Cell - Staurosporine Treated (24hr, 500nM)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 57 kDa). Abcam recommends using milk as the blocking agent.
IHC-P Use at an assay dependent concentration. PubMed: 24130898
IHC-Fr Use at an assay dependent concentration. PubMed: 21769484

ターゲット情報

  • 機能Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4.
  • 関連疾患Defects in PVRL1 are the cause of ectodermal dysplasia Margarita Island type (EDMI) [MIM:225060]; also known as Zlotogora-Ogur syndrome, cleft lip/palate-ectodermal dysplasia syndrome (CLPED1) or ectodermal dysplasia 4. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDMI is an autosomal recessive syndrome characterized by the association of cleft lip/palate, ectodermal dysplasia (sparse short and dry scalp hair, sparse eyebrows and eyelashes), and partial syndactyly of the fingers and/or toes. Two thirds of the patients do not manifest oral cleft but present with abnormal teeth and nails.
    Defects in PVRL1 are the cause of non-syndromic orofacial cleft type 7 (OFC7) [MIM:225060]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • 配列類似性Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD111 antibody
    • CD111 antigen antibody
    • CLPED1 antibody
    • ectodermal dysplasia 4 (Margarita Island type) antibody
    • ED4 antibody
    • Herpes virus entry mediator C antibody
    • Herpesvirus entry mediator C antibody
    • Herpesvirus Ig like receptor antibody
    • Herpesvirus Ig-like receptor antibody
    • HIgR antibody
    • HveC antibody
    • MGC142031 antibody
    • Nectin 1 antibody
    • Nectin-1 antibody
    • OFC7 antibody
    • OROFACIAL CLEFT 7 antibody
    • OTTHUMP00000232093 antibody
    • OTTHUMP00000232094 antibody
    • OTTHUMP00000232095 antibody
    • Poliovirus receptor related protein 1 antibody
    • poliovirus receptor-like 1 antibody
    • Poliovirus receptor-related protein 1 antibody
    • PRR antibody
    • PRR1 antibody
    • PVRL 1 antibody
    • PVRL1 antibody
    • PVRL1_HUMAN antibody
    • PVRR antibody
    • PVRR1 antibody
    • SK-12 antibody
    see all

Anti-PVRL1 antibody 画像

  • All lanes : Anti-PVRL1 antibody (ab66985) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse Dorsal Root Ganglion
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 210 kDa,35 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    PVRL1 protein is known to be glycosylated and it migrates at a higher molecular weight than the predicted band size of 57kDa (Swiss-prot data), as demonstrated by PMID 15731277. Abcam recommends using 3% milk as the blocking agent with ab66985
  • IHC-FoFr image of PVRL1 (ab66985) staining on Rat hippocampus and Striatum sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

Anti-PVRL1 antibody (ab66985) 使用論文

This product has been referenced in:
  • Gillory LA  et al. Preclinical evaluation of engineered oncolytic herpes simplex virus for the treatment of neuroblastoma. PLoS One 8:e77753 (2013). IHC-P ; Human . Read more (PubMed: 24130898) »
  • Lachke SA  et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet : (2011). IHC-Fr ; Mouse . Read more (PubMed: 21769484) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

投稿 Sep 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"