製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PUS1
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 377 and 427 of human PUS1 (NP_079491.2).

  • ポジティブ・コントロール
    • Hela whole cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab85938 was affinity purified using an epitope specific to PUS1 immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA.
  • 組織特異性
    Widely expressed. High levels of expression found in brain and skeletal muscle.
  • 関連疾患
    Defects in PUS1 are a cause of myopathy with lactic acidosis and sideroblastic anemia type 1 (MLASA1) [MIM:600462]; also known as mitochondrial myopathy and sideroblastic anemia. MLASA is a rare autosomal recessive oxidative phosphorylation disorder specific to skeletal muscle and bone marrow.
  • 配列類似性
    Belongs to the tRNA pseudouridine synthase TruA family.
  • 細胞内局在
    Mitochondrion and Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A730013B20Rik antibody
    • DOBI antibody
    • MGC112655 antibody
    • MGC11268 antibody
    • MLASA antibody
    • MLASA1 antibody
    • mPus1p antibody
    • Pseudouridine synthase 1 antibody
    • Pseudouridylate synthase 1 antibody
    • PUS1 antibody
    • tRNA pseudouridine synthase A, mitochondrial antibody
    • tRNA pseudouridine(38-40) synthase antibody
    • tRNA pseudouridylate synthase I antibody
    • tRNA uridine isomerase I antibody
    • tRNA-uridine isomerase I antibody
    • TRUA_HUMAN antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PUS1 with ab85938 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-PUS1 antibody (ab85938) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 47 kDa


    Exposure time : 10 secondsNote: there are two isoforms of this protein, the second missing amino acids 1-28, which could explain the presence of a double band.
  • Detection of human PUS1 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab85938 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

    Detection: Chemiluminescence with an exposure time of 10 seconds.
    Note: there are two isoforms of this protein, the second missing amino acids 1-28, which could explain the presence of a double band.

参考文献

ab85938 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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