製品の概要

  • 製品名Anti-Pumilio 2 antibody
    Pumilio 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Pumilio 2
  • アプリケーション適用あり: WB, IP, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human Pumilio homolog 2 encoded within exon 4 (LocusLink ID 23369).

  • ポジティブ・コントロール
    • Whole cell lysate from a human monocytic KG-1 cell line, mouse multipotential hematopoietic EML cell line or mouse pre-B 70 Z/3 cell line. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/20000. Detects a band of approximately 140 kDa (predicted molecular weight: 127 kDa).
IP Use at 5-10 µg/mg of lysate.
IHC-Fr Use at an assay dependent concentration. PubMed: 21865157
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
  • 組織特異性Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
  • 配列類似性Contains 1 PUM-HD domain.
    Contains 8 pumilio repeats.
  • ドメインThe pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
  • 細胞内局在Cytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ36528 antibody
    • KIAA0235 antibody
    • MGC138251 antibody
    • MGC138253 antibody
    • PUM 2 antibody
    • PUM2 antibody
    • PUM2_HUMAN antibody
    • PUMH 2 antibody
    • PUMH2 antibody
    • Pumilio (Drosphila) homolog 2 antibody
    • Pumilio homolog 2 (Drosophila) antibody
    • Pumilio homolog 2 antibody
    • Pumilio-2 antibody
    • Pumilio2 antibody
    • PUML2 antibody
    see all

Anti-Pumilio 2 antibody 画像



  • Predicted band size : 127 kDa


    ab10361 detection of human and mouse Pumilio 2 by Western blot. Samples: Whole cell lysate (50 mg) from a human monocytic (KG-1; lane 1), mouse multipotential hematopoietic (EML; lane 2) or mouse pre-B (70 Z/3; lane 3) cell lines. Antibody: ab10361 used at 0.1 mg/ml. Detection: Chemiluminescence with 3 to 5 minute exposure.
  • ICC/IF image of ab10361 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10361 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Pumilio 2 antibody (ab10361) 使用論文

This product has been referenced in:
  • Marrero E  et al. Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse. J Biol Chem 286:36492-9 (2011). WB, IHC-Fr ; Mouse . Read more (PubMed: 21865157) »
  • Vessey JP  et al. Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function. Proc Natl Acad Sci U S A 107:3222-7 (2010). WB . Read more (PubMed: 20133610) »

See all 4 Publications for this product

Product Wall

ab57644, anti-RPS23, code de remise : ******** ab10361, anti-Pumilio 2, code de remise : ******** Date d’expiration pour ces deux codes : JJ MM AAAA. Je suis ravi que vous souhaitiez participer à notre offre et tester ab10361 and ab57644 c...

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Thank you for contacting Abcam. As we do not know the exact immunogen sequence for ab10361 and the similarity between the human and sea urchin is not high, I would not suggest that particular antibody. I would however recommend trying ab57049, as we kn...

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Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control hnRNP C antibody (sc-15385) bound protein A dynabeads 0.1 mg/ml (antibody/beads).
Negative control Protein A dynabeads without antibody bound.
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投稿 Aug 18 2011

Thank you for your enquiry and your interest in our products. We do apologize but the immunizing peptides are not available commercially.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"