Anti-Pumilio 1 抗体 [EPR3795] (ab92545)


  • 製品名Anti-Pumilio 1 antibody [EPR3795]
    Pumilio 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3795] to Pumilio 1
  • 特異性This antibody is predicted to not cross react with Pumilio 2.
  • アプリケーション適用あり: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide. within Human Pumilio 1 aa 150-250. The exact sequence is proprietary.
    Database link: Q14671

  • ポジティブ・コントロール
    • WB: HeLa and 293T cell lysates IHC-P: Human fetal kidney tissue ICC/IF: HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab92545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 126 kDa.
IP 1/50.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.


Anti-Pumilio 1 antibody [EPR3795] 画像

  • All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysates

    Lysates/proteins at 10 µg per lane.

    Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 126 kDa
  • Immunohistochemical analysis of Pumilio 1 in paraffin embedded Human fetal kidney tissue using ab92545 at a 1/100 dilution.
  • Immunofluorescent staining of Pumilio 1 in HeLa cells using ab92545 at a 1/100 dilution.
  • Overlay histogram showing HEK293 cells stained with ab92545 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92545, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Pumilio 1 antibody [EPR3795] (ab92545) 使用論文

ab92545 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control hnRNP C antibody (sc-15386) bound protein A dynabeads 0.1 mg/ml (antibody/beads).
Negative control Protein A dynabeads without antibody bound.
Immuno-precipitation step Other - Protein A dynabeads

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投稿 Aug 18 2011