製品の概要

  • 製品名Anti-PUMA antibody [EP512Y]
    PUMA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP512Y] to PUMA
  • 特異性This antibody is specific for both the alpha and beta isoforms of PUMA. Isoform B is primarily recognized on Western blot gel.
  • アプリケーション適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C-terminal of Human PUMA.

  • ポジティブ・コントロール
    • K562 cell lysate, human breast carcinoma tissue, human ovary carcinoma tissue and Hela cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab33906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/30 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/5000. Detects a band of approximately 18-24 kDa (predicted molecular weight: 21 kDa).Can be blocked with PUMA peptide (ab206123).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.

ターゲット情報

  • 機能Essential mediator of p53-dependent and p53-independent apoptosis.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Belongs to the Bcl-2 family.
  • 細胞内局在Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BBC 3 antibody
    • Bbc3 antibody
    • BBC3_HUMAN antibody
    • BCL 2 binding component 3 antibody
    • Bcl-2-binding component 3 antibody
    • BCL2 binding component 3 antibody
    • JFY 1 antibody
    • JFY-1 antibody
    • JFY1 antibody
    • p53 up regulated modulator of apoptosis antibody
    • p53 up-regulated modulator of apoptosis antibody
    • p53 Upregulated Modulator of Apoptosis antibody
    • PUMA alpha antibody
    • PUMA/JFY1 antibody
    see all

Anti-PUMA antibody [EP512Y] 画像

  • ab33906 at a 1:250 dilution staining PUMA in Hela cells using Immunocytochemistry/Immunofluorescence.
  • ab33906 at a 1:100 dilution staining PUMA in human ovary carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
  • Anti-PUMA antibody [EP512Y] (ab33906) at 1/5000 dilution + K562 cell lysate.

    Predicted band size : 21 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
  • ab33906 at a 1:100 dilution staining PUMA in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
  • Overlay histogram showing K562 cells stained with ab33906 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33906, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in K562 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

Anti-PUMA antibody [EP512Y] (ab33906) 使用論文

This product has been referenced in:
  • Spurlock CF  et al. Methotrexate-mediated inhibition of nuclear factor ?B activation by distinct pathways in T cells and fibroblast-like synoviocytes. Rheumatology (Oxford) N/A:N/A (2014). Read more (PubMed: 25118313) »
  • Wilson AM  et al. ASPP1/2 regulate p53-dependent death of retinal ganglion cells through PUMA and Fas/CD95 activation in vivo. J Neurosci 33:2205-16 (2013). WB ; Rat, Mouse . Read more (PubMed: 23365256) »

See all 10 Publications for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - other (Mec1 (lymphocytic chronic leukemia))
Total protein in input 2e+007 cells
Immuno-precipitation step Protein G
Specification Mec1 (lymphocytic chronic leukemia)
Username

Dr. Isabel Marzo

Verified customer

投稿 Apr 18 2016

ab33906 lot YH090207C - 0.3260 mg/ml

ab33862 lot YH082410D - 0.6380 mg/ml

ab32429 lot YG080503 - 0.0790 mg/ml

ab40660 lot YI070802CS - 0.1030 mg/ml

ab32445 lot YI072804DS - 0.0250 mg/ml

Thank you for your enquiry.

1) The concentration for the lot YH090207C is 0.3260mg/ml.

2) The lab typically uses 1XPBS as a negative control.



Please let us know if you have further questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Bone marrow CD34+ cells)
Specification Bone marrow CD34+ cells
Fixation Formaldehyde
Permeabilization Yes - ice-cold methanol drop-wise, then 30min at -20oC, then 0.1% Triton-X in PBS for 15min at room temperature
Gating Strategy eFlour780 fixative vaibility dye (eBioScience) negative for live cell population
Username

Dr. Bon Ham Yip

Verified customer

投稿 Jul 06 2012

Merci pour votre requête.
Nous n'avons pas mesurée l'affinité de cet anticorps. Je peux par contre vous rassurer que cet anticorps avait été testé en Western blot à une dilution de 1/1000 jusqu'à 1/5000 avec des bons résultats. De plus je peux vou...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7)
Loading amount 50 µg
Specification MCF7
Treatment 5-FU, 1mM for 24hrs
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C
Username

Abcam user community

Verified customer

投稿 Mar 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Jurkat)
Specification Jurkat
Preparation Cell harvesting/tissue preparation method: Spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight.
Sample buffer: Buffer composition at analysis was 0.1% sodium azide with FBS in phosphate buffered solution.
Fixation Paraformaldehyde
Permeabilization Yes - Triton X-100 and NP40
Gating Strategy Isolate cell population from plot of SSC-A / FSA-A
Username

Abcam user community

Verified customer

投稿 Sep 02 2011

Thank you for your patience in the matter. I am very sorry to hear that you are having problems with this product (ab33906). As stated in our Abpromise, we will be more than happy to give a free replacement vial or refund if a product was report...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"