製品の概要

  • 製品名Anti-PU.1/Spi1 antibody [EPR3159Y]
    PU.1/Spi1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3159Y] to PU.1/Spi1
  • アプリケーション適用あり: WB, Flow Cyt, IHC-P, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues on the C terminal of human PU.1/Spi1

  • ポジティブ・コントロール
    • U937 and TF-1 cell lysates. Human tonsil tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 31 kDa).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
    • 配列類似性Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • transcription factor spi1 antibody
      • 31 kDa Transforming Protein antibody
      • 31 kDa-transforming protein antibody
      • cb1086 antibody
      • Hematopoietic transcription factor PU.1 antibody
      • OF antibody
      • oncogene spi1 antibody
      • PU.1 antibody
      • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
      • SFPI1 antibody
      • si:by184l24.2 antibody
      • SPI 1 antibody
      • SPI 1 proto oncogene antibody
      • SPI A antibody
      • Spi1 antibody
      • SPI1_HUMAN antibody
      • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
      • Spleen focus forming virus proviral integration oncogene spi1 antibody
      • Transcription factor PU.1 antibody
      see all

    Anti-PU.1/Spi1 antibody [EPR3159Y] 画像

    • All lanes : Anti-PU.1/Spi1 antibody [EPR3159Y] (ab76542) at 1/1000 dilution

      Lane 1 : U937 cell lysates
      Lane 2 : TF-1 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 31 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    • ab76542, at 1/100 dilution, staining PU.1/Spi1 in paraffin-embedded human tonsil tissue by immunohistochemistry.

    Anti-PU.1/Spi1 antibody [EPR3159Y] (ab76542) 使用論文

    ab76542 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76542.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"