製品の概要

  • 製品名Anti-PU.1/Spi1 antibody [EPR3158Y]
    PU.1/Spi1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3158Y] to PU.1/Spi1
  • アプリケーション適用あり: IHC-Fr, IHC-P, WB, Flow Cytmore details
    適用なし: ICC/IF or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N terminal of human PU.1/Spi1

  • ポジティブ・コントロール
    • Daudi and U937 cell lysates. Human tonsil tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/5000 - 1/20000. Detects a band of approximately 42 kDa (predicted molecular weight: 31 kDa).
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for ICC/IF or IP.
  • ターゲット情報

    • 機能Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
    • 配列類似性Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • transcription factor spi1 antibody
      • 31 kDa Transforming Protein antibody
      • 31 kDa-transforming protein antibody
      • cb1086 antibody
      • Hematopoietic transcription factor PU.1 antibody
      • OF antibody
      • oncogene spi1 antibody
      • PU.1 antibody
      • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
      • SFPI1 antibody
      • si:by184l24.2 antibody
      • SPI 1 antibody
      • SPI 1 proto oncogene antibody
      • SPI A antibody
      • Spi1 antibody
      • SPI1_HUMAN antibody
      • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
      • Spleen focus forming virus proviral integration oncogene spi1 antibody
      • Transcription factor PU.1 antibody
      see all

    Anti-PU.1/Spi1 antibody [EPR3158Y] 画像

    • All lanes : Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) at 1/10000 dilution

      Lane 1 : Daudi cell lysates
      Lane 2 : U937 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 31 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    • ab76543, at 1/100 dilution, staining PU.1/Spi1 in paraffin-embedded human tonsil tissue by immunohistochemistry.
    • ab76543 showing positive staining in Normal spleen tissue.

    • ab76543 showing positive staining in Normal lung lymphocytes staining tissue.

    • ab76543 showing negative staining in Normal brain tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) 使用論文

    This product has been referenced in:
    • Vieyra-Garcia PA  et al. STAT3/5-Dependent IL9 Overexpression Contributes to Neoplastic Cell Survival in Mycosis Fungoides. Clin Cancer Res 22:3328-39 (2016). IHC-P ; Human . Read more (PubMed: 26851186) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Sample Human Tissue sections (Lymph node)
    Specification Lymph node
    Permeabilization No
    Fixative Acetone
    Username

    Abcam user community

    Verified customer

    投稿 Jul 15 2013

    DISCOUNT CODE: 42636
    Expiration date:15-Mar-13

    I am very pleased to hear a customer would like to accept our offer and test ab76543 in IHC-Fr. To redeem this offer, an Abreview needs to be submitted for IHC-Fr on human samples and this cod...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"