Anti-PTS/PTPS 抗体 (ab96104)
Key features and details
- Rabbit polyclonal to PTS/PTPS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PTS/PTPS antibody
PTS/PTPS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PTS/PTPS -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Recombinant fragment corresponding to Human PTS/PTPS aa 1-111.
Database link: NP_000308 -
ポジティブ・コントロール
- WB: Transfected HEK-293T and HeLa cells, H1299 cells and mouse stomach tissue lysate. IHC-P: Human hepatoma tissue; IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96104の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 16 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin. -
パスウェイ
Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3. -
関連疾患
Defects in PTS are the cause of BH4-deficient hyperphenylalaninemia type A (HPABH4A) [MIM:261640]; also called 6-pyruvoyl-tetrahydropterin synthase deficiency (PTS deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to PTS deficiency. HPABH4A is an autosomal recessive disorder characterized by depletion of the neurotransmitters dopamine and serotonin, and clinically by severe neurological symptoms unresponsive to the classic phenylalanine-low diet. -
配列類似性
Belongs to the PTPS family. -
翻訳後修飾
Phosphorylation of Ser-19 is required for maximal enzyme activity. - Information by UniProt
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参照データベース
- Entrez Gene: 5805 Human
- Entrez Gene: 19286 Mouse
- Entrez Gene: 29498 Rat
- GenBank: NP_000308.1 Human
- Omim: 612719 Human
- SwissProt: Q03393 Human
- SwissProt: Q9R1Z7 Mouse
- SwissProt: P27213 Rat
see all -
別名
- 6 pyruvoyl tetrahydrobiopterin synthase antibody
- 6 pyruvoyl tetrahydropterin synthase antibody
- 6 pyruvoyltetrahydropterin synthase antibody
see all
画像
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All lanes : Anti-PTS/PTPS antibody (ab96104) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 16 kDa15% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded human hepatoma tissue staining PTS with ab96104 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labeling PTS (green) with ab96104 at 1/100 dilution. Costained with Hoechst 33342 (Bottom Panel).
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All lanes : Anti-PTS/PTPS antibody (ab96104) at 1/500 dilution
Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : PTS/PTPS Knockout HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 16 kDa15% SDS-PAGE
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Anti-PTS/PTPS antibody (ab96104) at 1/1000 dilution + H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 16 kDa
15% SDS PAGE -
Anti-PTS/PTPS antibody (ab96104) at 1/1000 dilution + Mouse stomach tissue lysate at 50 µg
Predicted band size: 16 kDa15% SDS PAGE
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab96104 は論文での使用が確認できていません。