製品の概要
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製品名Anti-PTRF antibody
PTRF 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to PTRF
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由来種Rabbit
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アプリケーション適用あり: WB, IPmore details
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種交差性交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Opossum, Bat, Elephant
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免疫原
Synthetic peptide corresponding to a region between residue 125 and 175 of human PTRF (NP_036364.2)
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ポジティブ･コントロール
- HeLa whole cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab76919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 43 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
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関連疾患Congenital generalized lipodystrophy 4
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配列類似性Belongs to the PTRF/SDPR family.
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翻訳後修飾Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity.
Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent. -
細胞内局在Membrane > caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm > cytosol. Mitochondrion. Nucleus. Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co-localizes with CAV1 in lipid rafts in adipocytes.
- Information by UniProt
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参照データベース
- Entrez Gene: 284119 Human
- Omim: 603198 Human
- SwissProt: Q6NZI2 Human
- Unigene: 437191 Human
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別名
- 2310075E07Rik antibody
- AW546441 antibody
- Cavin 1 antibody
see all
画像
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Western blot - Anti-PTRF antibody (ab76919)All lanes : Anti-PTRF antibody (ab76919) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 43 kDa
Observed band size: 51 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 minutes -
Immunoprecipitation - Anti-PTRF antibody (ab76919)Western Blot of Immunprecipitate.
ab76919 at 1µg/ml (for WB) staining PTRF in HeLa whole cell lysate,
immunoprecipitated using ab76919 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
参考文献
ab76919 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"