製品の概要

  • 製品名Anti-PTPRS antibody
  • 製品の詳細
    Mouse monoclonal to PTPRS
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein: EPPRFIKEPK DQIGVSGGVA SFVCQATGDP KPRVTWNKKG KKVNSQRFET IEFDESAGAV LRIQPLRTPR DENVYECVAQ NSVGEITVHA KLTVLRGP, corresponding to amino acids 1-129 of Human PTPRS

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 217 kDa.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Interacts with LAR-interacting protein LIP.1.
  • 組織特異性Detected in all tissues tested except for placenta and liver.
  • 配列類似性Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.
    Contains 8 fibronectin type-III domains.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • 翻訳後修飾A cleavage occurs, separating the extracellular domain from the transmembrane segment. This process called 'ectodomain shedding' is thought to be involved in receptor desensitization, signal transduction and/or membrane localization.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Protein tyrosine phosphatase PTPsigma antibody
    • Protein tyrosine phosphatase receptor type S antibody
    • Protein tyrosine phosphatase receptor type sigma antibody
    • Ptprs antibody
    • PTPRS_HUMAN antibody
    • PTPSIGMA antibody
    • R PTP sigma antibody
    • R-PTP-S antibody
    • R-PTP-sigma antibody
    • Receptor-type tyrosine-protein phosphatase S antibody
    • Receptor-type tyrosine-protein phosphatase sigma antibody
    see all

Anti-PTPRS antibody 画像



  • Predicted band size : 217 kDa
  • Anti-PTPRS antibody (ab55640) + immunogen

    Predicted band size : 217 kDa
  • Overlay histogram showing HeLa cells stained with ab55640 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55640, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-PTPRS antibody (ab55640) 使用論文

ab55640 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (4-12% gel, MES buffer, 4xLaemmli Sample buffer)
Sample Mouse Cell lysate - whole cell (Brain lysate)
Specification Brain lysate
Treatment 4xLaemmli Sample buffer, 80C boil 10 minutes
Blocking step SuperBlocking as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 22°C
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投稿 Jul 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"