製品の概要

  • 製品名Anti-PTPN12 antibody
    PTPN12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PTPN12
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 375 and 425 of human protein PTPN12 (NP_002826.2)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and 293T cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab76936 was affinity purified using an epitope specific to PTPN12 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2 - 5 ug/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 88 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 配列類似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Protein tyrosine phosphatase G1 antibody
      • Protein tyrosine phosphatase non receptor type 12 antibody
      • Protein-tyrosine phosphatase G1 antibody
      • PTN12_HUMAN antibody
      • PTP PEST antibody
      • PTP-PEST antibody
      • PTPG1 antibody
      • Ptpn12 antibody
      • Tyrosine protein phosphatase non receptor type 12 antibody
      • Tyrosine-protein phosphatase non-receptor type 12 antibody
      see all

    Anti-PTPN12 antibody 画像

    • All lanes : Anti-PTPN12 antibody (ab76936) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 88 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds
    • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab76936 at 1 ug/ml (used at 3µg/mg lysate for IP).
      Lane 2: control IgG.
      Detection: Chemiluminescence with an exposure time of 1 second.

    Anti-PTPN12 antibody (ab76936) 使用論文

    ab76936 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76936.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"