製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PTP4A3
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PTP4A3.

    (Peptide available as ab93307.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the folloing human tissue lysates: Heart; Skeletal muscle. It also gave a positive result in HCT116 cell line in ICC/IF.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. May be involved in the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II.
  • 組織特異性
    Mainly expressed in cardiomyocytes and skeletal muscle; also found in pancreas. Consistently overexpressed in colon cancer metastasis.
  • 配列類似性
    Belongs to the protein-tyrosine phosphatase family.
    Contains 1 tyrosine-protein phosphatase domain.
  • 翻訳後修飾
    Farnesylated. Farnesylation is required for membrane targeting.
  • 細胞内局在
    Cell membrane. Early endosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Potentially prenylated protein tyrosine phosphatase antibody
    • PRL 3 antibody
    • PRL R antibody
    • PRL-3 antibody
    • PRL-R antibody
    • PRL3 antibody
    • PRLR antibody
    • Protein tyrosine phosphatase 4a3 antibody
    • Protein tyrosine phosphatase type IVA 3 antibody
    • Protein-tyrosine phosphatase 4a3 antibody
    • Protein-tyrosine phosphatase of regenerating liver 3 antibody
    • PTP 4A3 antibody
    • PTP4A3 antibody
    • TP4A3_HUMAN antibody
    see all

画像

  • All lanes : Anti-PTP4A3 antibody (ab82568) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 19 kDa
    Additional bands at : 24 kDa,45 kDa,77 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • IHC image of PTP4A3 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82568, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • All lanes : Anti-PTP4A3 antibody (ab82568) at 1 µg/ml

    Lane 1 : Recombinant Human PTP4A3 protein (ab87716) at 0.01 µg
    Lane 2 : Recombinant Human PTP4A3 protein (ab87716) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds
  • ICC/IF image of ab82568 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Gari HH  et al. Genome-wide functional genetic screen with the anticancer agent AMPI-109 identifies PRL-3 as an oncogenic driver in triple-negative breast cancers. Oncotarget 7:15757-71 (2016). WB, IHC . Read more (PubMed: 26909599) »

See 1 Publication for this product

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