Anti-PTP4A1 抗体 (ab1368)

製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to PTP4A1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Peptide with sequence KDSNGHRNNCCIQ, from C-Terminus of the protein sequence according to NP_003454

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: 1/2000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 21 kDa in HeLa and A549 lysates (predicted molecular weight: 20 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis.
    • 組織特異性
      Expressed in bone marrow, lymph nodes, T lymphocytes, spleen, thymus and tonsil. Overexpressed in tumor cell lines.
    • 配列類似性
      Belongs to the protein-tyrosine phosphatase family.
      Contains 1 tyrosine-protein phosphatase domain.
    • 発生段階
      Expressed in fetal liver.
    • 翻訳後修飾
      Farnesylated. Farnesylation is required for membrane targeting. Unfarnesylated forms are shifted into the nucleus.
    • 細胞内局在
      Cell membrane. Early endosome. Endoplasmic reticulum. Cytoplasm. Cytoplasm > cytoskeleton > spindle. And mitotic spindle.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HH 72 antibody
      • HH72 antibody
      • Hypothetical protein DKFZp779M0721 antibody
      • Phosphatase of regenerating liver 1 antibody
      • PRL 1 antibody
      • PRL-1 antibody
      • PRL1 antibody
      • Protein tyrosine phosphatase PTPCAAX1 antibody
      • Protein tyrosine phosphatase type IVA 1 antibody
      • Protein tyrosine phosphatase type IVA member 1 antibody
      • Protein-tyrosine phosphatase 4a1 antibody
      • Protein-tyrosine phosphatase of regenerating liver 1 antibody
      • Protein-tyrosine phosphatase, type 4A, 1 antibody
      • PTP(CAAXI) antibody
      • Ptp4a1 antibody
      • PTPCAAX1 antibody
      • TP4A1_HUMAN antibody
      see all

    画像

    • Anti-PTP4A1 antibody (ab1368) at 2 µg/ml + Human A549 lysate (35µg in RIPA buffer)

      Predicted band size : 20 kDa

    プロトコール

    参考文献

    ab1368 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (COS7 extract expressing HA-tagged PTP4A1.)
    Loading amount
    10 µg
    Specification
    COS7 extract expressing HA-tagged PTP4A1.
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Blocking One as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 09 2009

    Thank you for your enquiry. We have withdrawn ab1368from our product range. We do hope that this does not cause you too much inconvenience. Have you purchased this antibody? Have you done any experiments with it?

    Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we offer a replacement or reimbursement. We do not routinely offer free samples. We also offer a USD20 Amazon gift voucher to researchers who feedback information a...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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