製品の概要

  • 製品名Anti-PTP1B (phospho Y152) antibody
    PTP1B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PTP1B (phospho Y152)
  • 特異性Does not react with control cells or after alkaline phosphatase treatment of blots.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic phosphopeptide corresponding to amino acids surrounding Tyr 152 of Human PTP1B.

  • ポジティブ・コントロール
    • Pervanadate-treated Human A431 and Jurkat cells or Mouse SYF cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Cross adsorbed to a phospho-tyrosine peptide, then affinity purified with phospho PTP1B (Tyr 152) peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab88473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報ELISA: 1/2000.
    WB: 1/1000. Detects a band of approximately 50 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能May play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • 配列類似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • 翻訳後修飾Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • 細胞内局在Endoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    Anti-PTP1B (phospho Y152) antibody 画像

    • All lanes : Anti-PTP1B (phospho Y152) antibody (ab88473) at 1/1000 dilution (in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature)

      Lane 1 : Mouse SYF cSrc-transformed cells, untreated
      Lane 2 : Mouse SYF cSrc-transformed cells treated with pervanadate (1 mM for 30 minutes)


      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    Anti-PTP1B (phospho Y152) antibody (ab88473) 使用論文

    ab88473 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab88473.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"