製品の概要

  • 製品名
    Anti-PTP1B (phospho Y152) antibody
    PTP1B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PTP1B (phospho Y152)
  • 特異性
    Does not react with control cells or after alkaline phosphatase treatment of blots.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic phosphopeptide corresponding to amino acids surrounding Tyr 152 of Human PTP1B.

  • ポジティブ・コントロール
    • Pervanadate-treated Human A431 and Jurkat cells or Mouse SYF cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Cross adsorbed to a phospho-tyrosine peptide, then affinity purified with phospho PTP1B (Tyr 152) peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab88473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: 1/2000.
    WB: 1/1000. Detects a band of approximately 50 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      May play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • 配列類似性
      Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • 翻訳後修飾
      Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • 細胞内局在
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    画像

    • All lanes : Anti-PTP1B (phospho Y152) antibody (ab88473) at 1/1000 dilution (in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature)

      Lane 1 : Mouse SYF cSrc-transformed cells, untreated
      Lane 2 : Mouse SYF cSrc-transformed cells treated with pervanadate (1 mM for 30 minutes)


      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    プロトコール

    参考文献

    ab88473 has not yet been referenced specifically in any publications.

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab88473.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録