Anti-PTP1B (phospho S50) 抗体 [EP1838Y] (ab62320)

製品の概要

  • 製品名
    Anti-PTP1B (phospho S50) antibody [EP1838Y]
    PTP1B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1838Y] to PTP1B (phospho S50)
  • 特異性
    This antibody detects PTP1B when phosphorylated at Serine 50.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surround Serine 50 of human PTP1B.

  • ポジティブ・コントロール
    • Hela cell lysate.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab62320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

ターゲット情報

  • 機能
    May play an important role in CKII- and p60c-src-induced signal transduction cascades.
  • 配列類似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • 翻訳後修飾
    Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • PTP1B antibody
    • Non receptor tyrosine phosphatase 1 antibody
    • Protein phosphotyrosylphosphatase 1B antibody
    • Protein tyrosine phosphatase 1B antibody
    • Protein tyrosine phosphatase non receptor type 1 antibody
    • Protein tyrosine phosphatase placental antibody
    • Protein-tyrosine phosphatase 1B antibody
    • PTN1_HUMAN antibody
    • PTP 1B antibody
    • PTP-1B antibody
    • PTPN 1 antibody
    • PTPN1 antibody
    • Tyrosine protein phosphatase non receptor type 1 antibody
    • Tyrosine-protein phosphatase non-receptor type 1 antibody
    see all

画像

  • All lanes : Anti-PTP1B (phospho S50) antibody [EP1838Y] (ab62320) at 1/5000 dilution

    Lane 1 : Hela cell lysate - untreated cells
    Lane 2 : Hela cell lysate - treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

プロトコール

参考文献

ab62320 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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