Anti-PTP1B (phospho S50) 抗体 (ab88472)

製品の概要

  • 製品名
    Anti-PTP1B (phospho S50) antibody
    PTP1B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PTP1B (phospho S50)
  • 特異性
    ab88472 reactivity is observed as a weak singlet in control (untreated) cells and is not observed after lambda phosphatase treatment.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to amino acids surrounding Ser 50 of Human PTP1B

  • ポジティブ・コントロール
    • Lysate from Human A431 and Jurkat cells treated with Calyculin A.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: 1/2000.
    WB: 1/1000. Detects a band of approximately 50 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      May play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • 配列類似性
      Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • 翻訳後修飾
      Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • 細胞内局在
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    画像

    • All lanes : Anti-PTP1B (phospho S50) antibody (ab88472) at 1/1000 dilution

      Lane 1 : lysate from untreated Jurkat cells
      Lane 2 : lysate from Jurkat cells treated with calyculin A (100 nM for 30 minutes)


      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    プロトコール

    参考文献

    ab88472 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (muscle cells)
    Loading amount
    40 µg
    Specification
    muscle cells
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

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    Verified customer

    投稿 Mar 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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