Anti-PTP1B (phospho S378) 抗体 [EP1840Y] (ab76239)

製品の概要

  • 製品名Anti-PTP1B (phospho S378) antibody [EP1840Y]
    PTP1B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1840Y] to PTP1B (phospho S378)
  • 特異性ab76239 detects PTP1B phosphorylated on serine 378.
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho-specific peptide corresponding to residues surrounding serine 378 of human PTP1B

  • ポジティブ・コントロール
    • HeLa cytoplasmic or nuclear lysate treated with calyculin A Human breast carcinoma tissue
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ICC
  • 追加情報ICC: 1/100 - 1/250.
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.
    WB: 1/500000 - 1/1000000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能May play an important role in CKII- and p60c-src-induced signal transduction cascades.
    • 配列類似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • 翻訳後修飾Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
    • 細胞内局在Endoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • PTP1B antibody
      • Non receptor tyrosine phosphatase 1 antibody
      • Protein phosphotyrosylphosphatase 1B antibody
      • Protein tyrosine phosphatase 1B antibody
      • Protein tyrosine phosphatase non receptor type 1 antibody
      • Protein tyrosine phosphatase placental antibody
      • Protein-tyrosine phosphatase 1B antibody
      • PTN1_HUMAN antibody
      • PTP 1B antibody
      • PTP-1B antibody
      • PTPN 1 antibody
      • PTPN1 antibody
      • Tyrosine protein phosphatase non receptor type 1 antibody
      • Tyrosine-protein phosphatase non-receptor type 1 antibody
      see all

    Anti-PTP1B (phospho S378) antibody [EP1840Y] 画像

    • All lanes : Anti-PTP1B (phospho S378) antibody [EP1840Y] (ab76239) at 1/1000000 dilution

      Lane 1 : HeLa cell lysates - untreated
      Lane 2 : HeLa cell lysates - treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 50 kDa
      Observed band size : 50 kDa
    • ab76239, at a 1/50 dilution, staining human PTP1B (phospho S378) in breast carcinoma by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

    Anti-PTP1B (phospho S378) antibody [EP1840Y] (ab76239) 使用論文

    ab76239 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76239.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"