Anti-PTP1B 抗体 [EP1841Y] (ab75856)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1841Y] to PTP1B
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PTP1B antibody [EP1841Y]
PTP1B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1841Y] to PTP1B -
由来種
Rabbit -
特異性
ab75856 detects both phosphorylated and nonphosphorylated PTP1B. -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa, HepG2 or Jurkat whole cell lysate (ab7899). IHC-P: Human tonsil tissue. Flow Cyt (intra): Jurkat cells. ICC/IF: HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1841Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75856の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/500.
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特記事項 |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/500. |
ターゲット情報
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機能
May play an important role in CKII- and p60c-src-induced signal transduction cascades. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
翻訳後修飾
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5770 Human
- Omim: 176885 Human
- SwissProt: P18031 Human
- Unigene: 417549 Human
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別名
- PTP1B antibody
- Non receptor tyrosine phosphatase 1 antibody
- Protein phosphotyrosylphosphatase 1B antibody
see all
画像
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PTPB1 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab75856 observed at 54 kDa. Red - loading control, ab8245, observed at 37 kDa.ab75856 was shown to specifically react with PTP1B when PTP1B knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab75856 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PTP1B with purified ab75856 at 1/500 dilution (5.3 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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ab75856, at 1/50 dilution, staining PTP1B in human tonsil, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of Jurkat cells labelling PTP1B with ab75856 at 1/2000 (red). Cells were fixed with 90% methanol. ab150077, Alexa Fluorr® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG (ab172730). Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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All lanes : Anti-PTP1B antibody [EP1841Y] (ab75856) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab75856 は 4 報の論文で使用されています。
- Xu X et al. miR-125a-5p inhibits tumorigenesis in hepatocellular carcinoma. Aging (Albany NY) 11:7639-7662 (2019). PubMed: 31527306
- Radmanesh F et al. Optimization of miRNA delivery by using a polymeric conjugate based on deoxycholic acid-modified polyethylenimine. Int J Pharm 565:391-408 (2019). PubMed: 31085260
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Weidmann MD et al. Mena(INV) dysregulates cortactin phosphorylation to promote invadopodium maturation. Sci Rep 6:36142 (2016). PubMed: 27824079