Anti-PTP rho 抗体 [T20-AF3C7] (ab85333)

製品の概要

  • 製品名
    Anti-PTP rho antibody [T20-AF3C7]
    PTP rho 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [T20-AF3C7] to PTP rho
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 887-1169 of Human PTP rho purified from E.coli.

  • ポジティブ・コントロール
    • transfected Bosc23 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.03% Sodium Azide
    Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • 特記事項(精製)
    Purified from TCS
  • ポリ/モノ
    モノクローナル
  • クローン名
    T20-AF3C7
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Predicted molecular weight: 164 kDa.
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能
    May be involved in both signal transduction and cellular adhesion in the CNS.
  • 組織特異性
    Expressed in colon, lung, heart and testis, as well as in fetal and adult brain. Not detected in muscle and peripheral blood leukocytes.
  • 配列類似性
    Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
    Contains 4 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 MAM domain.
    Contains 2 tyrosine-protein phosphatase domains.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Protein tyrosine phosphatase receptor type T antibody
    • PTPRT antibody
    • PTPRT_HUMAN antibody
    • R PTP T antibody
    • R-PTP-T antibody
    • Receptor protein tyrosine phosphatase antibody
    • Receptor-type tyrosine-protein phosphatase rho antibody
    • Receptor-type tyrosine-protein phosphatase T antibody
    • RPTP-rho antibody
    • RPTPmam4 antibody
    • RPTPrho antibody
    see all

画像

  • Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175



    All lanes : Anti-NF-kB p65 (phospho S529) antibody (ab84333) at 1/5000 dilution

    Lane 1 : 293T Cell Lysate
    Lane 2 : Precipitated from 293T Cell Lysate 200ug using ab84333 2ug
    Lane 3 : Precipitated from 293T Cell Lysate 200ug using ab84333 5ug
    Lane 4 : Precipitated from PBS using ab84333 5ug
  • All lanes : Anti-PTP rho antibody [T20-AF3C7] (ab85333) at 1/10000 dilution

    Lane 1 : Bosc23 Cell Lysate
    Lane 2 : Bosc23 Cell Lysate Transfected with Myc/DUSP12
    Lane 3 : Bosc23 Cell Lysate Transfected with Myc/R-PTP rho
    Lane 4 : Bosc23 Cell Lysate Transfected with GFP/Ub

    Predicted band size: 164 kDa



    Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

  • All lanes : Anti-PTP rho antibody [T20-AF3C7] (ab85333) at 1/5000 dilution

    Lane 1 : Bosc23 cell lysate
    Lane 2 : Bosc23 cell lysate transfected with Myc/His/Tau (1-441 aa)
    Lane 3 : Bosc23 cell lysate transfected with Myc/His/PTP rho (877-1169 aa)
    Lane 4 : Bosc23 cell lysate transfected with GFP-Ub+1

    Predicted band size: 164 kDa



    The observed band size may not correspond to the predicted protein molecular weight as a recombinant fragment was used for the test lane.

参考文献

ab85333 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your enquiry and your interest in our product.
I can confirm that the immunogen used to raise this antibody was a recombinant fragment, corresponding to amino acids 887-1169 of Human PTP rho purified from E.coli.
I hope this hel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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