製品の概要

  • 製品名
    Anti-PTP rho antibody [AFT20]
    PTP rho 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AFT20] to PTP rho
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human PTP rho.

  • ポジティブ・コントロール
    • Bosc23 cell transfected with Myc/His/R PTP rho.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.03% Sodium azide
    Constituents: 0.87% Sodium chloride, HEPES, 0.01% BSA, 50% Glycerol
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • ポリ/モノ
    モノクローナル
  • クローン名
    AFT20
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 164 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    May be involved in both signal transduction and cellular adhesion in the CNS.
  • 組織特異性
    Expressed in colon, lung, heart and testis, as well as in fetal and adult brain. Not detected in muscle and peripheral blood leukocytes.
  • 配列類似性
    Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
    Contains 4 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 MAM domain.
    Contains 2 tyrosine-protein phosphatase domains.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Protein tyrosine phosphatase receptor type T antibody
    • PTPRT antibody
    • PTPRT_HUMAN antibody
    • R PTP T antibody
    • R-PTP-T antibody
    • Receptor protein tyrosine phosphatase antibody
    • Receptor-type tyrosine-protein phosphatase rho antibody
    • Receptor-type tyrosine-protein phosphatase T antibody
    • RPTP-rho antibody
    • RPTPmam4 antibody
    • RPTPrho antibody
    see all

画像

  • All lanes : Anti-PTP rho antibody [AFT20] (ab75521) at 1/10000 dilution

    Lane 1 : Bosc23 Cell Lysate
    Lane 2 : Bosc23 Cell Lysate Transfected with Myc/MKP2
    Lane 3 : Bosc23 Cell Lysate Transfected with Myc/R-PTP rho
    Lane 4 : Bosc23 Cell Lysate Transfected with GFP/Ub


    Predicted band size : 164 kDa
  • All lanes : Anti-PTP rho antibody [AFT20] (ab75521) at 1/2000 dilution

    Lane 1 : Bosc23 cell lysate
    Lane 2 : Bosc23 cell lysate transfected with Myc/His/R PTP rho (887 - 1169 aa)


    Predicted band size : 164 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab75521 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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