製品の概要

  • 製品名Anti-PTGIS antibody
    PTGIS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PTGIS
  • アプリケーション適用あり: ICC/IF, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Cow, Human
  • 免疫原

    Synthetic peptide:

    PEFDLSRYGFGLMQPE

    , corresponding to amino acids 475-490 of Mouse Prostaglandin I synthase (PTGIS).

アプリケーション

Our Abpromise guarantee covers the use of ab23668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/250. Predicted molecular weight: 57 kDa.Can be blocked with PTGIS peptide (ab187257).

ターゲット情報

Anti-PTGIS antibody 画像

  • ab 23668 at 1/200 staining primary mouse myoblasts by ICC/IF.

    The tissue section was formaldehyde fixed, and permeabilized and blocked with 100% TNB buffer.  The blocking time was 1 hour, at 25°C.

    The primary antibody was incubated for 16 hours at 4°C.

    The secondary antibody was a biotin conjugated Donkey anti-Rabbit IgG polyclonal diluted1/800.

    See Abreview

  • ab23668 (4µg/ml) staining PTGIS in human adrenal gland using an automated system (DAKO Autostainer Plus). Using this protocol there is apical cytoplasmic/ endoplasmic reticulum staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-PTGIS antibody (ab23668) 使用論文

This product has been referenced in:
  • Cipollone F  et al. A unique microRNA signature associated with plaque instability in humans. Stroke 42:2556-63 (2011). Read more (PubMed: 21817153) »
  • Singh SR  et al. Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways? Am J Physiol Lung Cell Mol Physiol 299:L169-83 (2010). ICC/IF ; Human . Read more (PubMed: 20435685) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Pig Tissue lysate - whole (Carotid artery)
Loading amount 30 µg
Specification Carotid artery
Gel Running Conditions Reduced Non-Denaturing (Native) (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Apr 09 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Primary mouse myoblasts)
Specification Primary mouse myoblasts
Fixative Formaldehyde
Permeabilization Yes - TNB buffer, Perkin Elmer
Blocking step TNB buffer, Perkin Elmer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Nov 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"