Anti-PTEN (phospho T366) 抗体 [EP229] (ab109454)

製品の概要

• 製品名
  Anti-PTEN (phospho T366) antibody [EP229]
  PTEN 一次抗体 製品一覧
• 製品の詳細
  Rabbit monoclonal [EP229] to PTEN (phospho T366)
• 特異性
  ab109454 only detects PTEN phosphorylated on T366.
• アプリケーション
  適用あり: WB, IP, IHC-Pmore details
  適用なし: Flow Cyt
• 種交差性
  交差種: Mouse, Rat, Human
  
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PTEN (phospho T366).

• ポジティブ･コントロール
  • WB: Mouse brain tissue lysate and C6 cell lysate treated with Calyculin A. IHC-P: Human breast ductal carcinoma tissue.
• 特記事項

  This product is a recombinant rabbit monoclonal antibody.

   

   

  Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
• バッファー
  PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• 精製度
  Tissue culture supernatant
• ポリ/モノ
  モノクローナル
• クローン名
  EP229
• アイソタイプ
  IgG
• 研究分野
  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Phosphatases

  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • Lipid Phosphatases

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Tumor Suppressors

  • Cancer
  • Cell cycle
  • Kinases/phosphatases
  • Phosphatases

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • PTEN pathway

  • Metabolism
  • Types of disease
  • Diabetes

  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Types of disease
  • Metabolic disorders

アプリケーション

ターゲット情報

• 機能
  Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement.
  Isoform alpha: Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.
• 組織特異性
  Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
• 関連疾患
  Cowden syndrome 1
  Lhermitte-Duclos disease
  Bannayan-Riley-Ruvalcaba syndrome
  Squamous cell carcinoma of the head and neck
  Endometrial cancer
  PTEN mutations are found in a subset of patients with Proteus syndrome, a genetically heterogeneous condition. The molecular diagnosis of PTEN mutation positive cases classifies Proteus syndrome patients as part of the PTEN hamartoma syndrome spectrum. As such, patients surviving the early years of Proteus syndrome are likely at a greater risk of developing malignancies.
  Glioma 2
  VACTERL association with hydrocephalus
  Prostate cancer
  Macrocephaly/autism syndrome
  A microdeletion of chromosome 10q23 involving BMPR1A and PTEN is a cause of chromosome 10q23 deletion syndrome, which shows overlapping features of the following three disorders: Bannayan-Zonana syndrome, Cowden disease and juvenile polyposis syndrome.
• 配列類似性
  Contains 1 C2 tensin-type domain.
  Contains 1 phosphatase tensin-type domain.
• ドメイン
  The C2 domain binds phospholipid membranes in vitro in a Ca(2+)-independent manner; this binding is important for its tumor suppressor function.
• 翻訳後修飾
  Constitutively phosphorylated by CK2 under normal conditions. Phosphorylated in vitro by MAST1, MAST2, MAST3 and STK11. Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both inhibit or promote PDZ-binding. Phosphorylation at Tyr-336 by FRK/PTK5 protects this protein from ubiquitin-mediated degradation probably by inhibiting its binding to NEDD4. Phosphorylation by ROCK1 is essential for its stability and activity. Phosphorylation by PLK3 promotes its stability and prevents its degradation by the proteasome.
  Monoubiquitinated; monoubiquitination is increased in presence of retinoic acid. Deubiquitinated by USP7; leading to its nuclear exclusion. Monoubiquitination of one of either Lys-13 and Lys-289 amino acid is sufficient to modulate PTEN compartmentalization. Ubiquitinated by XIAP/BIRC4.
• 細胞内局在
  Secreted. May be secreted via a classical signal peptide and reenter into cells with the help of a poly-Arg motif and Cytoplasm. Nucleus. Nucleus, PML body. Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies. XIAP/BIRC4 promotes its nuclear localization.

• Target information above from: UniProt accession P60484 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 5728 Human
  • Entrez Gene: 19211 Mouse
  • Entrez Gene: 50557 Rat
  • Omim: 601728 Human
  • SwissProt: P60484 Human
  • SwissProt: O08586 Mouse
  • Unigene: 500466 Human
  • Unigene: 729457 Human

  • Unigene: 245395 Mouse
  • Unigene: 22158 Rat

  see all
• 別名
  • 10q23del antibody
  • BZS antibody
  • DEC antibody

  • GLM2 antibody
  • MGC11227 antibody
  • MHAM antibody
  • MMAC1 antibody
  • MMAC1 phosphatase and tensin homolog deleted on chromosome 10 antibody
  • Mutated in multiple advanced cancers 1 antibody
  • Phosphatase and tensin homolog antibody
  • Phosphatase and tensin like protein antibody
  • Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN antibody
  • Pten antibody
  • PTEN_HUMAN antibody
  • PTEN1 antibody
  • TEP1 antibody

  see all

プロトコール

• Western blot protocols
• Immunoprecipitation protocols
• Immunohistochemistry protocols