製品の概要

  • 製品名
    Anti-PTCHD1 antibody
  • 製品の詳細
    Goat polyclonal to PTCHD1
  • 由来種
    Goat
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-DFQKTSRVSER

    , corresponding to internal sequence amino acids 260-270 of Human PTCHD1 (NP_775766.2).

  • ポジティブ・コントロール
    • Human Brain (Cerebellum) lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 101 kDa).

ターゲット情報

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  • Anti-PTCHD1 antibody (ab109407) at 0.5 µg/ml + Human Brain Cerebellum lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 101 kDa



    An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide.

プロトコール

参考文献

ab109407 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (cerebellum, kidney)
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
70 µg
Specification
cerebellum, kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mike Liu

Verified customer

投稿 Apr 17 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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