製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to PTBP1
  • アプリケーション
    適用あり: IP, IHC-P, ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human PTBP1 aa 2-14 (N terminal).
    Sequence:

    DGIVPDIAVGTKR-C


    Database link: P26599
    (Peptide available as ab23105)

  • ポジティブ・コントロール
    • Jurkat lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration. PubMed: 17967866
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60-65 kDa (predicted molecular weight: 61-64 kDa).Can be blocked with Human PTBP1 peptide (ab23105).
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:32,000.

ターゲット情報

  • 機能
    Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
  • 配列類似性
    Contains 4 RRM (RNA recognition motif) domains.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 57 kDa RNA binding protein PPTB 1 antibody
    • 57 kDa RNA-binding protein PPTB-1 antibody
    • Heterogeneous nuclear ribonucleoprotein I antibody
    • Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody
    • hnRNP I antibody
    • HNRNP-I antibody
    • HNRNPI antibody
    • HNRPI antibody
    • MGC10830 antibody
    • MGC8461 antibody
    • Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody
    • Polypyrimidine Tract Binding Protein 1 antibody
    • Polypyrimidine tract binding protein antibody
    • Polypyrimidine tract-binding protein 1 antibody
    • pPTB antibody
    • PTB 1 antibody
    • PTB 2 antibody
    • PTB 3 antibody
    • PTB 4 antibody
    • PTB antibody
    • PTB T antibody
    • PTB1 antibody
    • PTB2 antibody
    • PTB3 antibody
    • PTB4 antibody
    • PTBP 1 antibody
    • PTBP1 antibody
    • PTBP1_HUMAN antibody
    • PTBT antibody
    • RNA Binding Protein antibody
    see all

画像

  • Immunohistochemical analysis of paraffin embedded Human Kidney tissue labeling PTBP1 with ab5642 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.

  • All lanes : Anti-PTBP1 antibody (ab5642) at 0.01 µg/ml

    Lane 1 : Human epithelial cells from cervix adenocarcinoma
    Lane 2 : Human liver hepatocellular carcinoma
    Lane 3 : Human epithelial cells from embryonic kidney

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 61-64 kDa
  • Immunohistochemical analysis of paraffin embedded Human Kidney tissue labeling PTBP1 with ab5642 at 2 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.



  • Predicted band size : 61-64 kDa

    ab5642 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

    ab5642 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ICC/IF image of ab5642 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5642, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab5642 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5642, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-PTBP1 antibody (ab5642) at 0.1 µg/ml + Human Ovary Lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 61-64 kDa
  • ab5642 (5µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining shows nuclear staining of keratinocytes.

参考文献

This product has been referenced in:
  • Kalantari R  et al. Stable association of RNAi machinery is conserved between the cytoplasm and nucleus of human cells. RNA 22:1085-98 (2016). IP ; Human . Read more (PubMed: 27198507) »
  • Fiesel FC  et al. (Patho-)physiological relevance of PINK1-dependent ubiquitin phosphorylation. EMBO Rep 16:1114-30 (2015). Read more (PubMed: 26162776) »

See all 7 Publications for this product

レビューと Q&A

Thank you for the details that you have provided. At this point, I would like to make the following suggestions. Use ab5642 at a concentration of 1 µg/ml and incubate overnight at 4C. Do not re-use the primary antibody. Ensure that your secondary antib...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab5642. A secondary antibody that is compatible for use with this primary is ab6741 - Rabbit polyclonal to Goat IgG H&L (HRP). I would like to help you out an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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