製品の概要

  • 製品名Anti-PSTPIP1 antibody
    PSTPIP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PSTPIP1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length Human protein (AAH08602)

  • ポジティブ・コントロール
    • Raw 264.7 cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab88474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Involved in regulation of the actin cytoskeleton. May regulate the WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to the ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allows PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation (By similarity). Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2.
  • 組織特異性Highly expressed in the peripheral blood leukocytes, granulocytes and monocytes, namely in T-cells and natural killer cells, and in spleen. Weakly expressed in the thymus, small intestine, lung and placenta.
  • 関連疾患Defects in PSTPIP1 are the cause of PAPA syndrome (PAPAS) [MIM:604416]; also known as pyogenic sterile arthritis, pyoderma gangrenosum and acne or familial recurrent arthritis (FRA). PAPAS is characterized by autosomal dominant inheritance of early onset, primarily affecting skin and joint tissues. Recurring inflammatory episodes lead to accumulation of sterile, pyogenic, neutrophil-rich material within the affected joints, ultimately resulting in significant destruction.
  • 配列類似性Contains 1 FCH domain.
    Contains 1 SH3 domain.
  • ドメインThe coiled domain mediates interaction with PTPN18, PTPN12 and CD2AP. The SH3 domain mediates interaction with WAS and ABL1 (By similarity). The SH3 and coiled-coil domains are necessary for the interaction with MEFV.
  • 翻訳後修飾Dephosphorylated on Tyr-345 by PTPN18, this event negatively regulates the association of PSTPIP1 with SH2 domain-containing proteins as tyrosine kinase. Phosphorylation of Tyr-345 is probably required for subsequent phosphorylation at other tyrosine residues. Phosphorylation is induced by activation of the EGFR and PDGFR in a ABL1 dependent manner. The phosphorylation regulates the interaction with WAS and with MEFV.
  • 細胞内局在Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cytoplasm > perinuclear region. Cleavage furrow. Colocalized with the cortical actin cytoskeleton during interphase, lamellipodia and actin-rich cytokinetic cleavage furrow. Colocalized with WAS to filamentous structures within the cytoplasm. Colocalized with PTPN12 in the cytoplasm and the perinuclear region. Colocalized with CD2AP and WAS in the actin cytoskeleton. Colocalized with CD2, CD2AP and WAS at the site of T-cell:APC contact.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD2 antigen binding protein 1 antibody
    • CD2 binding protein 1 antibody
    • CD2 cytoplasmic tail binding protein antibody
    • CD2-binding protein 1 antibody
    • CD2BP1 antibody
    • CD2BP1L antibody
    • CD2BP1S antibody
    • H PIP antibody
    • H-PIP antibody
    • HPIP antibody
    • PAPAS antibody
    • PEST phosphatase interacting protein 1 antibody
    • PEST phosphatase-interacting protein 1 antibody
    • PPIP1_HUMAN antibody
    • Proline serine threonine phosphatase interacting protein 1 antibody
    • Proline-serine-threonine phosphatase-interacting protein 1 antibody
    • PSTPIP antibody
    • Pstpip1 antibody
    see all

Anti-PSTPIP1 antibody 画像

  • Anti-PSTPIP1 antibody (ab88474) at 1 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 48 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)
  • Anti-PSTPIP1 antibody (ab88474) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 48 kDa

Anti-PSTPIP1 antibody (ab88474) 使用論文

ab88474 has not yet been referenced specifically in any publications.

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