製品の概要

  • 製品名Anti-PSME1 antibody
    PSME1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PSME1
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human PSME1 aa 220-249 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q06323

  • ポジティブ・コントロール
    • HL60 cell line lysates; Human hepatocarcinoma tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab104098 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab104098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use 5µg for 10 cells.
WB 1/100 - 1/500. Predicted molecular weight: 29 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
  • 配列類似性Belongs to the PA28 family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 11S regulator complex alpha subunit antibody
    • 11S regulator complex subunit alpha antibody
    • 29 kD MCP activator subunit antibody
    • 29kD MCP activator subunit antibody
    • Activator of multicatalytic protease subunit 1 antibody
    • IFI5111 antibody
    • IGUP I-5111 antibody
    • Interferon gamma IEF SSP 5111 antibody
    • Interferon gamma inducible protein 5111 antibody
    • Interferon gamma up regulated I 5111 protein antibody
    • Interferon gamma up-regulated I-5111 protein antibody
    • MGC8628 antibody
    • PA28a antibody
    • PA28alpha antibody
    • Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) antibody
    • Proteasome activator 28 subunit alpha antibody
    • Proteasome activator complex subunit 1 antibody
    • Proteasome activator subunit 1 antibody
    • PSME1 antibody
    • PSME1_HUMAN antibody
    • REG-alpha antibody
    • REGalpha antibody
    see all

Anti-PSME1 antibody 画像

  • ICC/IF image of ab104098 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104098 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PSME1 antibody (ab104098) at 1/100 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 29 kDa
  • ab104098 at 1/50 dilution, staining PMSE1 in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-PSME1 antibody (ab104098) 使用論文

ab104098 has not yet been referenced specifically in any publications.

Product Wall

Gracias por contactarnos.

El anticuerpo ab104098 se ha purificado mediante columna de proteína A, seguido de purificación por afinidad con el inmunógeno inmovilizado en la columna.

Espero que esta informaci&oacu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"