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RabMAb

Anti-PSMD14 抗体 [EPR4257] (ab109123)

製品の概要

  • 製品名
    Anti-PSMD14 antibody [EPR4257]
    PSMD14 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4257] to PSMD14
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human PSMD14.

  • ポジティブ・コントロール
    • HepG2, A431, HeLa, Namalwa, K562 and MCF7 cell lysates; Human colon tissue; HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).
ICC/IF 1/500 - 1/1000.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
    • 組織特異性
      Widely expressed. Highest levels in heart and skeletal muscle.
    • 配列類似性
      Belongs to the peptidase M67A family. PSMD14 subfamily.
      Contains 1 MPN (JAB/Mov34) domain.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 26S proteasome non-ATPase regulatory subunit 14 antibody
      • 26S proteasome regulatory subunit rpn11 antibody
      • 26S proteasome-associated PAD1 homolog 1 antibody
      • 26S proteasome-associated PAD1 homolog antibody
      • PAD1 antibody
      • PAD1, yeast, homolog of antibody
      • POH1 antibody
      • Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody
      • Proteasome 26S subunit non ATPase 14 antibody
      • PSDE_HUMAN antibody
      • Psmd14 antibody
      • RPN11 antibody
      • Testis tissue sperm binding protein Li 69n antibody
      see all

    Anti-PSMD14 antibody [EPR4257] 画像

    • All lanes : Anti-PSMD14 antibody [EPR4257] (ab109123) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : Namalwa cell lysates
      Lane 5 : K562 cell lysates
      Lane 6 : MCF7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
    • ab109123, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
    • ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.

    Anti-PSMD14 antibody [EPR4257] (ab109123) 使用論文

    ab109123 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Purified protein (protein purified from yeast or recombinantly expre)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycine Biorad Stain free)
    Loading amount
    0.4 µg
    Specification
    protein purified from yeast or recombinantly expre
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
    Username

    Ellen Goodall

    Verified customer

    投稿 Aug 01 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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