The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1:12500.
機能Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
組織特異性Widely expressed. Highest levels in heart and skeletal muscle.
配列類似性Belongs to the peptidase M67A family. PSMD14 subfamily. Contains 1 MPN (JAB/Mov34) domain.