Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.
Belongs to the proteasome subunit S9 family. Contains 1 PCI domain.
All lanes : Anti-PSMD11 antibody (ab99414) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 3 minutes
Immunoprecipitation - PSMD11 antibody (ab99414)
Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99414 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: PSMD11 antibody (ab99414) at 0.4 µg/ml