Rabbit polyclonal to PSMD1
Mouse, Rat, Chicken, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PSMD1.
- This antibody gave a positive signal in the following whole cell lysates: K562; HeLa; A431; HepG2; HEK293.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 2 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Belongs to the proteasome subunit S1 family.
Contains 10 PC repeats.
Information by UniProt
- MGC133041 antibody
- 26S proteasome non ATPase regulatory subunit 1 antibody
- 26S proteasome non ATPase subunit 1 antibody
Western blot - Anti-PSMD1 antibody (ab93208)
All lanes :
Anti-PSMD1 antibody (ab93208) at 2 µg/mlLane 1 :
K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 2 :
HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 :
A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 5 :
HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.SecondaryAll lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
106 kDaObserved band size:
106 kDaAdditional bands at:
75 kDa. We are unsure as to the identity of these extra bands.Exposure time:
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"