製品の概要

  • 製品名Anti-PSMD1 antibody
    PSMD1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to PSMD1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Fusion protein:

    IKETKENDEEFYKNKYSSKPYKVDNMTRILPQQSRYISFIKDDRFVPVRK FKGNNGVVVLRDREPKEPVALIETVRQMKDVNAPLPTPFKVDDNVDFPSA

    , corresponding to amino acids 846/945 of Saccharomyces cerevisiae Rpn2

  • 特記事項Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーConstituents: 50% Glycerol
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab21638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 104 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • 配列類似性Belongs to the proteasome subunit S1 family.
    Contains 10 PC repeats.
  • Information by UniProt
  • 別名
    • MGC133041 antibody
    • 26S proteasome non ATPase regulatory subunit 1 antibody
    • 26S proteasome non ATPase subunit 1 antibody
    • 26S proteasome non-ATPase regulatory subunit 1 antibody
    • 26S proteasome regulatory subunit rpn2 antibody
    • 26S proteasome regulatory subunit S1 antibody
    • 26S proteasome subunit p112 antibody
    • MGC133040 antibody
    • P112 antibody
    • Proteasome (prosome macropain) 26S subunit, non ATPase 1 antibody
    • Psmd1 antibody
    • PSMD1_HUMAN antibody
    • RPN2 antibody
    • S1 antibody
    see all

Anti-PSMD1 antibody 画像

  • All lanes : Anti-PSMD1 antibody (ab21638) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 104 kDa

Anti-PSMD1 antibody (ab21638) 使用論文

This product has been referenced in:
  • Kuzina E  et al. Glatiramer Acetate and Nanny Proteins Restrict Access of the Multiple Sclerosis Autoantigen Myelin Basic Protein to the 26S Proteasome. Biomed Res Int 2014:926394 (2014). WB . Read more (PubMed: 25276831) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"