The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).
By Western blot, this antibody detects a 42 kDa protein representing proteasome 19S subunit S10B from HeLa cell lysate. This antibody detects, to a lesser extent, an ~30 kDa protein which could correspond to subunit degradation product.
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.