製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PSMC3
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 389-439 of Human PSMC3 (NP_002795.2).

  • ポジティブ・コントロール
    • 293T, NIH 3T3, Jurkat and HeLa whole cell lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能
    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.
  • 配列類似性
    Belongs to the AAA ATPase family.
  • 翻訳後修飾
    Sumoylated by UBE2I in response to MEKK1-mediated stimuli.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 26S protease regulatory subunit 6A antibody
    • 26S proteasome AAA-ATPase subunit RPT5 antibody
    • Human immunodeficiency virus tat transactivator binding protein 1 antibody
    • MGC8487 antibody
    • Proteasome (prosome macropain) 26S subunit ATPase 3 antibody
    • Proteasome 26S ATPase subunit 3 antibody
    • Proteasome 26S subunit ATPase 3 antibody
    • Proteasome subunit P50 antibody
    • PRS6A_HUMAN antibody
    • PSMC 3 antibody
    • PSMC3 antibody
    • Tat binding protein 1 antibody
    • TAT-binding protein 1 antibody
    • TBP-1 antibody
    • TBP1 antibody
    see all

画像

  • All lanes : Anti-PSMC3 antibody (ab125164) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 49 kDa


    Exposure time : 30 seconds
  • ab125164, at 6 µg/mg lysate, staining PSMC3 in 293T cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane).
    For the subsequent Western blot, ab125164 was used at 1 µg/ml, and the blot developed using the ECL technique, with an exposure time of 10 seconds.

参考文献

ab125164 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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