The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報WB: Use at a concentration of 1-5 µg/ml. IF/ICC: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
配列類似性Belongs to the peptidase T1A family.
細胞内局在Cytoplasm. Nucleus. Cytoplasm, P-body. Colocalizes with TRIM5 in the cytoplasmic bodies.
PSMA4 antibody (ab55625) used in immunofluorescence at 1-5ug/ml on HeLa cells.
Western blot - Anti-PSMA4 antibody (ab55625)
Predicted band size : 29 kDa PSMA4 antibody (ab55625) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Anti-PSMA4 antibody (ab55625) 使用論文
This product has been referenced in:
Levy-Barda A et al. Involvement of the nuclear proteasome activator PA28? in the cellular response to DNA double-strand breaks. Cell Cycle10:4300-10 (2011).
Read more (PubMed: 22134242) »
Ben-Aroya S et al. Proteasome nuclear activity affects chromosome stability by controlling the turnover of mms22, a protein important for DNA repair. PLoS Genet6:e1000852 (2010).
Read more (PubMed: 20174551) »